Drug Information

Drug (ID: DG00491) and It's Reported Resistant Information

• Name: Huaier extract

Type(s) of Resistant Mechanism of This Drug

  EADR: Epigenetic Alteration of DNA, RNA or Protein

Drug Resistance Data Categorized by Their Corresponding Diseases

ICD-02: Benign/in-situ/malignant neoplasm

Breast cancer [ICD-11: 2C60]

Drug Sensitivity Data Categorized by Their Corresponding Mechanisms

Epigenetic Alteration of DNA, RNA or Protein (EADR)

Key Molecule: H19, imprinted maternally expressed transcript (H19) [1]

• Molecule Alteration: Expression; Down-regulation
• Sensitive Disease: Breast cancer [ICD-11: 2C60.3]
• Experimental Note: Revealed Based on the Cell Line Data
• Cell Pathway Regulation: LncRH19/MiR675-5p signaling pathway; Regulation; hsa05206
• In Vitro Model: MCF-7 cells; Breast; Homo sapiens (Human); CVCL_0031
• In Vitro Model: MDA-MB-231 cells; Breast; Homo sapiens (Human); CVCL_0062
• In Vitro Model: MDA-MB-468 cells; Breast; Homo sapiens (Human); CVCL_0419
• Experiment for Molecule Alteration: qRT-PCR
• Experiment for Drug Resistance: Flow cytometry assay; MTT assay
• Mechanism Description: miR675-5p was identified as a mature product of H19. The overexpression of H19 or miR675-5p reverses the tumor-inhibitory effects of Huaier extract. However, knocking down H19 or miR675-5p sensitizes breast cancer cells to Huaier extract. Huaier extract inhibits breast cancer cell proliferation and induces apoptosis via the LncRNA H19/miR675-5p/CBL signaling pathway.

Key Molecule: hsa-miR-675-5p [1]

• Molecule Alteration: Expression; Down-regulation
• Sensitive Disease: Breast cancer [ICD-11: 2C60.3]
• Experimental Note: Revealed Based on the Cell Line Data
• Cell Pathway Regulation: LncRH19/MiR675-5p signaling pathway; Regulation; hsa05206
• In Vitro Model: MCF-7 cells; Breast; Homo sapiens (Human); CVCL_0031
• In Vitro Model: MDA-MB-231 cells; Breast; Homo sapiens (Human); CVCL_0062
• In Vitro Model: MDA-MB-468 cells; Breast; Homo sapiens (Human); CVCL_0419
• Experiment for Molecule Alteration: qPCR
• Experiment for Drug Resistance: Flow cytometry assay; MTT assay
• Mechanism Description: miR675-5p was identified as a mature product of H19. The overexpression of H19 or miR675-5p reverses the tumor-inhibitory effects of Huaier extract. However, knocking down H19 or miR675-5p sensitizes breast cancer cells to Huaier extract. Huaier extract inhibits breast cancer cell proliferation and induces apoptosis via the LncRNA H19/miR675-5p/CBL signaling pathway.

References

• Ref 1: Huaier Extract Inhibits Breast Cancer Progression Through a LncRNA-H19/MiR-675-5p Pathway. Cell Physiol Biochem. 2017;44(2):581-593. doi: 10.1159/000485093. Epub 2017 Nov 17.