HIV Detail Information

Darunavir (ID: DG00044) Resistance Data of HIV

Drug Resistance Data Categorized by Their Corresponding Mechanisms

Aberration of the Drug's Therapeutic Target (ADTT)

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Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.I15V+p.V32I+p.L33F+p.L63P+p.C67F+p.A71V+p.G73S+p.I84V+p.L90M+p.I93L	Missense mutation	13	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, I15V, V32I, L33F, L63P, C67F, A71V, G73S, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I3F+p.L10I+p.K20R+p.V32I+p.L33F+p.E34K+p.E35D+p.M36I+p.N37D+p.R41K+p.K43E+p.K45R+p.I54M+p.D60K+p.L63P+p.A71V+p.I72T+p.V77I+p.I84V+p.L90M+p.I93L	Missense mutation	97	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I3F, L10I, K20R, V32I, L33F, E34K, E35D, M36I, N37D, R41K, K43E, K45R, I54M, D60K, L63P, A71V, I72T, V77I, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.G16GA+p.K20KR+p.V32I+p.L33F+p.M36MIL+p.N37ND+p.M46I+p.I47V+p.I54M+p.Q58E+p.L63P+p.A71V+p.V82A+p.I93L	Missense mutation	81	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, G16GA, K20KR, V32I, L33F, M36MIL, N37ND, M46I, I47V, I54M, Q58E, L63P, A71V, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13IV+p.L33F+p.N37S+p.R41K+p.K43R+p.M46ML+p.I54V+p.K55R+p.R57K+p.I62V+p.L63P+p.I64IM+p.A71V+p.G73S+p.V82C+p.L89V+p.L90M+p.I93L	Missense mutation	16	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13IV, L33F, N37S, R41K, K43R, M46ML, I54V, K55R, R57K, I62V, L63P, I64IM, A71V, G73S, V82C, L89V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13M+p.K20I+p.M36I+p.N37D+p.M46I+p.I54V+p.I62V+p.L63P+p.I66IV+p.C67F+p.A71T+p.I72T+p.G73T+p.I84V+p.L90M+p.I93L	Missense mutation	26	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13M, K20I, M36I, N37D, M46I, I54V, I62V, L63P, I66IV, C67F, A71T, I72T, G73T, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13M+p.L19LI+p.K20I+p.M36I+p.N37S+p.M46I+p.I54V+p.I62V+p.L63P+p.I64IV+p.I66V+p.C67F+p.H69HQ+p.I72IT+p.G73T+p.I84V+p.L90M+p.I93L	Missense mutation	15	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13M, L19LI, K20I, M36I, N37S, M46I, I54V, I62V, L63P, I64IV, I66V, C67F, H69HQ, I72IT, G73T, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.D30N+p.L33F+p.K45R+p.I54L+p.L63P+p.I66IV+p.P79PS+p.I84V+p.N88D+p.L89LF+p.L90M+p.I93L+p.C95CF	Missense mutation	51	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, D30N, L33F, K45R, I54L, L63P, I66IV, P79PS, I84V, N88D, L89LF, L90M, I93L, C95CF.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.G16A+p.L19V+p.L33F+p.E34Q+p.K43I+p.M46L+p.G51A+p.I54M+p.L63P+p.I64M+p.A71V+p.I72M+p.G73A+p.I84V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, G16A, L19V, L33F, E34Q, K43I, M46L, G51A, I54M, L63P, I64M, A71V, I72M, G73A, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.K14R+p.I15V+p.K20T+p.D30N+p.L33F+p.E35D+p.M36I+p.R41I+p.K43RS+p.I54L+p.R57K+p.I62V+p.L63P+p.I66V+p.A71V+p.I72T+p.V77I+p.N88D+p.L90M+p.Q92K+p.I93L+p.C95F+p.T96S	Missense mutation	16	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, K14R, I15V, K20T, D30N, L33F, E35D, M36I, R41I, K43RS, I54L, R57K, I62V, L63P, I66V, A71V, I72T, V77I, N88D, L90M, Q92K, I93L, C95F, T96S.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.K20I+p.L24I+p.M36I+p.M46I+p.I54V+p.D60E+p.L63P+p.I64V+p.I66V+p.A71T+p.I72T+p.G73C+p.I84V+p.T91S	Missense mutation	15	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, K20I, L24I, M36I, M46I, I54V, D60E, L63P, I64V, I66V, A71T, I72T, G73C, I84V, T91S.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.K20I+p.L24I+p.V32VI+p.M36I+p.M46I+p.I54V+p.D60E+p.I64V+p.I66V+p.A71F+p.I72T+p.G73C+p.I84V+p.T91S	Missense mutation	12	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, K20I, L24I, V32VI, M36I, M46I, I54V, D60E, I64V, I66V, A71F, I72T, G73C, I84V, T91S.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.K20R+p.D30N+p.V32I+p.L33F+p.M36I+p.N37D+p.M46I+p.I54L+p.L63P+p.I64L+p.I66V+p.A71T+p.I72T+p.I84V+p.N88D+p.L89M+p.L90M+p.I93L+p.C95F	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, K20R, D30N, V32I, L33F, M36I, N37D, M46I, I54L, L63P, I64L, I66V, A71T, I72T, I84V, N88D, L89M, L90M, I93L, C95F.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.K20T+p.L24I+p.D30N+p.L33F+p.E35D+p.M36I+p.N37S+p.K43T+p.F53L+p.K55N+p.L63P+p.V77VI+p.N88D+p.L89M	Missense mutation	15	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, K20T, L24I, D30N, L33F, E35D, M36I, N37S, K43T, F53L, K55N, L63P, V77VI, N88D, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.L19I+p.K20I+p.L33V+p.E35D+p.M36I+p.N37D+p.R41K+p.K45KR+p.M46I+p.I47A+p.F53Y+p.I62V+p.I64V+p.T74S+p.I84V+p.I85IV+p.L89I+p.L90M	Missense mutation	35	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, L19I, K20I, L33V, E35D, M36I, N37D, R41K, K45KR, M46I, I47A, F53Y, I62V, I64V, T74S, I84V, I85IV, L89I, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.L19I+p.K20T+p.V32I+p.E35N+p.M36I+p.M46I+p.G48Q+p.I54L+p.L63P+p.C67E+p.A71V+p.T74S+p.L76V+p.I84V+p.L89V+p.L90M+p.T91S+p.I93L	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, L19I, K20T, V32I, E35N, M36I, M46I, G48Q, I54L, L63P, C67E, A71V, T74S, L76V, I84V, L89V, L90M, T91S, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.L19I+p.K20R+p.L23I+p.E35D+p.M36I+p.R41K+p.M46I+p.I50V+p.F53L+p.I54V+p.L63P+p.I64V+p.I66V+p.A71I+p.G73T+p.L90M+p.I93L	Missense mutation	30	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, L19I, K20R, L23I, E35D, M36I, R41K, M46I, I50V, F53L, I54V, L63P, I64V, I66V, A71I, G73T, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.L19V+p.K20R+p.V32I+p.L33F+p.E35D+p.M36L+p.N37D+p.M46I+p.I54M+p.R57K+p.I62V+p.L63P+p.I66T+p.A71I+p.I72R+p.G73C+p.V77I+p.I84V+p.L89V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, L19V, K20R, V32I, L33F, E35D, M36L, N37D, M46I, I54M, R57K, I62V, L63P, I66T, A71I, I72R, G73C, V77I, I84V, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.L33F+p.E35D+p.M36I+p.I54L+p.I62V+p.L63P+p.A71V+p.G73S+p.I84V+p.L90M	Missense mutation	29	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, L33F, E35D, M36I, I54L, I62V, L63P, A71V, G73S, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.T12P+p.I13V+p.L24F+p.L33F+p.E34Q+p.N37S+p.R41K+p.M46I+p.I54V+p.R57G+p.I62V+p.L63P+p.A71V+p.I72IT+p.G73S+p.P79D+p.I84V+p.L90M+p.I93L	Missense mutation	17	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, T12P, I13V, L24F, L33F, E34Q, N37S, R41K, M46I, I54V, R57G, I62V, L63P, A71V, I72IT, G73S, P79D, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.I13IV+p.K20R+p.V32I+p.L33F+p.E35D+p.M36I+p.N37Q+p.M46I+p.I47V+p.I54L+p.R57K+p.Q58E+p.D60E+p.I62V+p.L63P+p.A71V+p.G73S+p.V77T+p.I84V+p.L89V+p.L90M+p.I93L	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, I13IV, K20R, V32I, L33F, E35D, M36I, N37Q, M46I, I47V, I54L, R57K, Q58E, D60E, I62V, L63P, A71V, G73S, V77T, I84V, L89V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.I13V+p.G16A+p.L19LV+p.L33F+p.E34Q+p.K43I+p.M46L+p.G51A+p.I54M+p.L63P+p.I64M+p.C67Y+p.A71V+p.I72M+p.G73A+p.I84V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, I13V, G16A, L19LV, L33F, E34Q, K43I, M46L, G51A, I54M, L63P, I64M, C67Y, A71V, I72M, G73A, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.I13V+p.I15V+p.G16A+p.K20I+p.L23I+p.E34D+p.E35K+p.M36I+p.N37D+p.K43T+p.M46I+p.I47IV+p.G51A+p.F53L+p.K55R+p.R57G+p.I62V+p.L63P+p.A71I+p.G73S+p.L76V+p.L89V+p.L90M+p.T91S	Missense mutation	82	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, I13V, I15V, G16A, K20I, L23I, E34D, E35K, M36I, N37D, K43T, M46I, I47IV, G51A, F53L, K55R, R57G, I62V, L63P, A71I, G73S, L76V, L89V, L90M, T91S.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.I13V+p.L19Q+p.K20M+p.V32I+p.L33V+p.E35A+p.M36I+p.M46I+p.I47V+p.I54M+p.R57K+p.I62V+p.L63P+p.I64V+p.G73T+p.T74A+p.I84V+p.L89V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, I13V, L19Q, K20M, V32I, L33V, E35A, M36I, M46I, I47V, I54M, R57K, I62V, L63P, I64V, G73T, T74A, I84V, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.I13V+p.V32I+p.L33F+p.E34Q+p.N37S+p.K43KT+p.M46L+p.I54L+p.Q61E+p.I62V+p.L63P+p.A71IV+p.T74P+p.V77I+p.I84V+p.N88G+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, I13V, V32I, L33F, E34Q, N37S, K43KT, M46L, I54L, Q61E, I62V, L63P, A71IV, T74P, V77I, I84V, N88G, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.I15V+p.K20V+p.E34QR+p.E35D+p.M36I+p.N37S+p.R41K+p.G48I+p.I54L+p.K55R+p.Q58E+p.D60NS+p.I62V+p.L63P+p.A71I+p.I72IM+p.G73S+p.L76V+p.I84V+p.L90M+p.I93L	Missense mutation	45	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, I15V, K20V, E34QR, E35D, M36I, N37S, R41K, G48I, I54L, K55R, Q58E, D60NS, I62V, L63P, A71I, I72IM, G73S, L76V, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.T12P+p.I13V+p.I15V+p.L19P+p.K20I+p.E35G+p.M36I+p.M46I+p.I54V+p.L63P+p.A71V+p.I72L+p.G73S+p.I84V+p.L90M+p.I93L	Missense mutation	11	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, T12P, I13V, I15V, L19P, K20I, E35G, M36I, M46I, I54V, L63P, A71V, I72L, G73S, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.T12P+p.I13V+p.I15V+p.L19P+p.K20T+p.V32I+p.L33F+p.E35G+p.M36I+p.I54IV+p.I62V+p.L63P+p.K70T+p.A71I+p.G73S+p.P79A+p.I84V+p.L89V+p.L90M	Missense mutation	100	High-level resistance	[2]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, T12P, I13V, I15V, L19P, K20T, V32I, L33F, E35G, M36I, I54IV, I62V, L63P, K70T, A71I, G73S, P79A, I84V, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.T12P+p.I13V+p.I15V+p.L19P+p.K20T+p.V32I+p.L33F+p.E35G+p.M36I+p.I54V+p.I62V+p.L63P+p.K70T+p.A71I+p.G73S+p.P79A+p.I84V+p.L89V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, T12P, I13V, I15V, L19P, K20T, V32I, L33F, E35G, M36I, I54V, I62V, L63P, K70T, A71I, G73S, P79A, I84V, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.T12P+p.I13V+p.K20I+p.L24M+p.E35D+p.M36I+p.M46I+p.Q61QR+p.I62V+p.L63E+p.C67Y+p.A71I+p.I72T+p.G73S+p.T74P+p.P79A+p.I84V+p.L89V+p.L90M+p.Q92K+p.I93L	Missense mutation	42	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, T12P, I13V, K20I, L24M, E35D, M36I, M46I, Q61QR, I62V, L63E, C67Y, A71I, I72T, G73S, T74P, P79A, I84V, L89V, L90M, Q92K, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11I+p.T12P+p.I13V+p.L19Q+p.K20M+p.V32I+p.E35EG+p.M36IT+p.K43T+p.M46I+p.I47V+p.I54M+p.K55R+p.R57G+p.L63P+p.C67CY+p.H69Q+p.A71I+p.V82A+p.L89V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11I, T12P, I13V, L19Q, K20M, V32I, E35EG, M36IT, K43T, M46I, I47V, I54M, K55R, R57G, L63P, C67CY, H69Q, A71I, V82A, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11VI+p.I13V+p.G16A+p.K20T+p.L33LEQ+p.E35EG+p.M46I+p.K55KR+p.R57K+p.Q61H+p.L63P+p.A71V+p.G73S+p.L76V+p.V77VI+p.I84V+p.L89V+p.L90M+p.I93L	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11VI, I13V, G16A, K20T, L33LEQ, E35EG, M46I, K55KR, R57K, Q61H, L63P, A71V, G73S, L76V, V77VI, I84V, L89V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11VI+p.T12TA+p.I13V+p.K14KR+p.K20R+p.D30N+p.V32I+p.L33F+p.E35D+p.M36V+p.N37T+p.K43Q+p.M46I+p.I47V+p.F53L+p.I54L+p.K55KR+p.R57K+p.I62V+p.L63P+p.I66IV+p.C67W+p.A71V+p.I72V+p.V77I+p.I84V+p.I85IL+p.N88D+p.L89LV+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11VI, T12TA, I13V, K14KR, K20R, D30N, V32I, L33F, E35D, M36V, N37T, K43Q, M46I, I47V, F53L, I54L, K55KR, R57K, I62V, L63P, I66IV, C67W, A71V, I72V, V77I, I84V, I85IL, N88D, L89LV, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V11VL+p.I13IV+p.V32I+p.L33F+p.K43T+p.M46I+p.I47V+p.F53L+p.I54L+p.K55KR+p.D60DE+p.I62V+p.L63P+p.I66L+p.C67CW+p.A71T+p.I72T+p.G73C+p.V77I+p.V82C+p.L90M+p.I93L	Missense mutation	80	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V11VL, I13IV, V32I, L33F, K43T, M46I, I47V, F53L, I54L, K55KR, D60DE, I62V, L63P, I66L, C67CW, A71T, I72T, G73C, V77I, V82C, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V32I+p.L33F+p.M36L+p.N37S+p.R41RK+p.M46I+p.I54L+p.K55R+p.R57K+p.I62V+p.L63P+p.I64V+p.I66V+p.G73I+p.I84V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V32I, L33F, M36L, N37S, R41RK, M46I, I54L, K55R, R57K, I62V, L63P, I64V, I66V, G73I, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.V32I+p.L33F+p.P39PS+p.M46MI+p.I47V+p.L63P+p.A71I+p.I72KR+p.G73S+p.I84V+p.L90M	Missense mutation	54	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, V32I, L33F, P39PS, M46MI, I47V, L63P, A71I, I72KR, G73S, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10FI+p.K20R+p.L33F+p.E35D+p.M36I+p.N37D+p.I54V+p.I62V+p.L63P+p.I64L+p.A71V+p.G73T+p.V82VI+p.I84V+p.L90M+p.I93L	Missense mutation	17	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10FI, K20R, L33F, E35D, M36I, N37D, I54V, I62V, L63P, I64L, A71V, G73T, V82VI, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.G16A+p.L19Q+p.V32I+p.N37S+p.R41K+p.K45R+p.M46I+p.I47A+p.L63P+p.A71V+p.I72V+p.V82A+p.L90M+p.Q92K+p.I93L	Missense mutation	11	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, G16A, L19Q, V32I, N37S, R41K, K45R, M46I, I47A, L63P, A71V, I72V, V82A, L90M, Q92K, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13IV+p.V32I+p.L33F+p.K43KT+p.I54L+p.L63P+p.A71V+p.G73T+p.V77I+p.P79A+p.I84V+p.L90M	Missense mutation	49	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13IV, V32I, L33F, K43KT, I54L, L63P, A71V, G73T, V77I, P79A, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.G16A+p.K20KR+p.L24F+p.V32I+p.L33F+p.M36MI+p.R41RK+p.K43T+p.M46I+p.F53L+p.K55KR+p.L63P+p.A71V+p.T74P+p.V77VI+p.V82A+p.L90M+p.I93L	Missense mutation	62	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, G16A, K20KR, L24F, V32I, L33F, M36MI, R41RK, K43T, M46I, F53L, K55KR, L63P, A71V, T74P, V77VI, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.I15IV+p.L19V+p.L33F+p.K43KT+p.K45KR+p.I54V+p.I62IV+p.L63P+p.A71V+p.T74P+p.V82L+p.I84V+p.L90M+p.I93IM	Missense mutation	10	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, I15IV, L19V, L33F, K43KT, K45KR, I54V, I62IV, L63P, A71V, T74P, V82L, I84V, L90M, I93IM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.I15V+p.K20R+p.L24I+p.L33F+p.E35D+p.M36I+p.N37E+p.K45R+p.G48GA+p.F53FI+p.I54V+p.R57K+p.Y59YC+p.I62V+p.L63P+p.G68E+p.A71T+p.I72T+p.G73C+p.V82A+p.I93L	Missense mutation	15	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, I15V, K20R, L24I, L33F, E35D, M36I, N37E, K45R, G48GA, F53FI, I54V, R57K, Y59YC, I62V, L63P, G68E, A71T, I72T, G73C, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.I15V+p.K20T+p.V32I+p.L33F+p.E35EK+p.I47V+p.R57G+p.L63P+p.I66F+p.A71V+p.G73S+p.V77I+p.L89V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, I15V, K20T, V32I, L33F, E35EK, I47V, R57G, L63P, I66F, A71V, G73S, V77I, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.I15V+p.L19V+p.L24I+p.V32I+p.L33F+p.K43E+p.M46L+p.I54L+p.D60E+p.L63P+p.A71V+p.I72V+p.V82A+p.I84V	Missense mutation	46	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, I15V, L19V, L24I, V32I, L33F, K43E, M46L, I54L, D60E, L63P, A71V, I72V, V82A, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20R+p.L24I+p.L33F+p.E34V+p.E35D+p.M36I+p.N37E+p.G48M+p.I50V+p.F53L+p.I54A+p.R57G+p.D60E+p.I62V+p.L63P+p.A71V+p.V77I+p.V82A+p.I93L	Missense mutation	33	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20R, L24I, L33F, E34V, E35D, M36I, N37E, G48M, I50V, F53L, I54A, R57G, D60E, I62V, L63P, A71V, V77I, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20R+p.L33F+p.E35D+p.M36I+p.N37E+p.K43T+p.M46I+p.I54V+p.R57K+p.D60E+p.Q61E+p.I62V+p.L63P+p.I64IV+p.G68E+p.A71T+p.I72T+p.G73AT+p.V82F+p.L90M+p.I93L	Missense mutation	32	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20R, L33F, E35D, M36I, N37E, K43T, M46I, I54V, R57K, D60E, Q61E, I62V, L63P, I64IV, G68E, A71T, I72T, G73AT, V82F, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20R+p.V32VI+p.L33F+p.E34N+p.E35D+p.M36I+p.N37ND+p.P39S+p.R41K+p.K43T+p.I47V+p.I54M+p.I62V+p.L63PT+p.I64V+p.A71T+p.T74S+p.V77I+p.V82T+p.I84V+p.I85IV+p.L90M+p.I93L	Missense mutation	23	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20R, V32VI, L33F, E34N, E35D, M36I, N37ND, P39S, R41K, K43T, I47V, I54M, I62V, L63PT, I64V, A71T, T74S, V77I, V82T, I84V, I85IV, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L19V+p.L24I+p.V32I+p.L33F+p.K43E+p.M46L+p.I54V+p.D60E+p.L63P+p.A71V+p.I72V+p.V82A+p.I84V	Missense mutation	27	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L19V, L24I, V32I, L33F, K43E, M46L, I54V, D60E, L63P, A71V, I72V, V82A, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L24I+p.L33F+p.E35D+p.M36I+p.N37S+p.M46I+p.I54M+p.L63P+p.A71V+p.V82VI+p.I84V+p.I93L	Missense mutation	27	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L24I, L33F, E35D, M36I, N37S, M46I, I54M, L63P, A71V, V82VI, I84V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L24I+p.V32VI+p.L33F+p.N37ND+p.M46I+p.I54L+p.I62IV+p.L63P+p.A71V+p.G73V+p.T74P+p.V82A+p.I84V+p.I93L	Missense mutation	24	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L24I, V32VI, L33F, N37ND, M46I, I54L, I62IV, L63P, A71V, G73V, T74P, V82A, I84V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L33F+p.E35D+p.M36I+p.P39PL+p.K43KT+p.F53PS+p.I54L+p.R57G+p.Q58E+p.I62V+p.A71V+p.T74S+p.V82F+p.L90M+p.I93L	Missense mutation	18	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L33F, E35D, M36I, P39PL, K43KT, F53PS, I54L, R57G, Q58E, I62V, A71V, T74S, V82F, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.V32I+p.L33F+p.N37S+p.P39PS+p.R41RK+p.I54L+p.R57G+p.L63PS+p.A71V+p.P79X+p.I84V	Missense mutation	35	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, V32I, L33F, N37S, P39PS, R41RK, I54L, R57G, L63PS, A71V, P79X, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15IV+p.K20R+p.E35D+p.M36I+p.M46L+p.I54V+p.Q58E+p.D60E+p.Q61E+p.I62IV+p.L63P+p.I64V+p.E65ED+p.I66IV+p.A71V+p.V82F+p.I84V+p.I85V+p.I93L	Missense mutation	41	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15IV, K20R, E35D, M36I, M46L, I54V, Q58E, D60E, Q61E, I62IV, L63P, I64V, E65ED, I66IV, A71V, V82F, I84V, I85V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.G17D+p.L19W+p.K20I+p.M36I+p.M46I+p.I47V+p.I54V+p.I62V+p.L63P+p.A71V+p.I72N+p.G73T+p.I84V+p.I85V+p.L90M+p.I93L	Missense mutation	13	Potential low-level resistance	[3]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, G17D, L19W, K20I, M36I, M46I, I47V, I54V, I62V, L63P, A71V, I72N, G73T, I84V, I85V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.K20KR+p.L33F+p.E34ED+p.M36MV+p.N37ND+p.M46I+p.I54MV+p.I62V+p.L63P+p.I66F+p.C67F+p.I72L+p.G73T+p.V75I+p.I84V+p.L90M+p.I93IM+p.C95V	Missense mutation	18	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, K20KR, L33F, E34ED, M36MV, N37ND, M46I, I54MV, I62V, L63P, I66F, C67F, I72L, G73T, V75I, I84V, L90M, I93IM, C95V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.E35D+p.M36I+p.N37E+p.G48V+p.I50V+p.I54A+p.R57RK+p.I62V+p.L63P+p.A71V+p.I72V+p.V82A+p.L90M+p.I93L	Missense mutation	14	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, E35D, M36I, N37E, G48V, I50V, I54A, R57RK, I62V, L63P, A71V, I72V, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.L33F+p.E34A+p.E35D+p.M36I+p.N37D+p.K43T+p.M46I+p.G48V+p.I50V+p.I54T+p.I62V+p.L63P+p.A71V+p.I72V+p.V77I+p.V82A+p.I85V+p.I93L	Missense mutation	54	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, L33F, E34A, E35D, M36I, N37D, K43T, M46I, G48V, I50V, I54T, I62V, L63P, A71V, I72V, V77I, V82A, I85V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.L33I+p.M36I+p.I54V+p.Q58E+p.D60E+p.L63P+p.A71V+p.V82L+p.I84V+p.L90M+p.I93L	Missense mutation	20	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, L33I, M36I, I54V, Q58E, D60E, L63P, A71V, V82L, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.V32I+p.L33F+p.E35D+p.M36I+p.N37D+p.R41IK+p.M46I+p.I47V+p.I54V+p.I62V+p.L63P+p.A71V+p.I72V+p.G73T+p.P79S+p.I84V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, V32I, L33F, E35D, M36I, N37D, R41IK, M46I, I47V, I54V, I62V, L63P, A71V, I72V, G73T, P79S, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K43S+p.M46I+p.I54V+p.R57K+p.I62V+p.L63P+p.A71V+p.G73T+p.V77I+p.V82F+p.L90M+p.I93L	Missense mutation	10	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K43S, M46I, I54V, R57K, I62V, L63P, A71V, G73T, V77I, V82F, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L19I+p.K20R+p.E34D+p.E35D+p.M36I+p.R41K+p.M46I+p.I47V+p.I54M+p.L63P+p.A71T+p.I84V+p.I85V+p.L90M+p.I93L	Missense mutation	36	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L19I, K20R, E34D, E35D, M36I, R41K, M46I, I47V, I54M, L63P, A71T, I84V, I85V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L19I+p.K20R+p.E35D+p.M36I+p.K43KE+p.M46L+p.G48V+p.I54CST+p.R57K+p.Q58E+p.I62V+p.L63P+p.A71V+p.I72V+p.V82A+p.I93L	Missense mutation	10	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L19I, K20R, E35D, M36I, K43KE, M46L, G48V, I54CST, R57K, Q58E, I62V, L63P, A71V, I72V, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L33F+p.M46I+p.F53L+p.I54L+p.I62V+p.L63P+p.A71V+p.T74P+p.I84V+p.L90M+p.I93L	Missense mutation	28	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L33F, M46I, F53L, I54L, I62V, L63P, A71V, T74P, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L33F+p.M46I+p.I54V+p.L63P+p.A71V+p.T74P+p.I84V+p.L90M+p.I93L	Missense mutation	16	Low-level resistance	[3]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L33F, M46I, I54V, L63P, A71V, T74P, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.N37X+p.M46I+p.I54ILM+p.I62V+p.L63P+p.I64V+p.A71V+p.I72IT+p.L76V+p.V77I+p.I84V+p.I85V+p.G86GR+p.I93L	Missense mutation	13	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, N37X, M46I, I54ILM, I62V, L63P, I64V, A71V, I72IT, L76V, V77I, I84V, I85V, G86GR, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12N+p.I13V+p.L33F+p.M46L+p.I54IV+p.Q61QH+p.L63P+p.I64V+p.A71V+p.T74P+p.V77I+p.V82F+p.I84V+p.L90M+p.I93L	Missense mutation	53	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12N, I13V, L33F, M46L, I54IV, Q61QH, L63P, I64V, A71V, T74P, V77I, V82F, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12P+p.I15V+p.K20R+p.V32VI+p.M36I+p.N37D+p.M46L+p.I54L+p.L63P+p.A71V+p.T74TS+p.P79S+p.V82VA+p.I84V+p.I85V+p.L90M+p.I93L	Missense mutation	62	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12P, I15V, K20R, V32VI, M36I, N37D, M46L, I54L, L63P, A71V, T74TS, P79S, V82VA, I84V, I85V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12P+p.L19V+p.L33F+p.M46L+p.G48M+p.I54M+p.L63T+p.A71V+p.I72T+p.T74A+p.V82A+p.I84V+p.I93L	Missense mutation	17	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12P, L19V, L33F, M46L, G48M, I54M, L63T, A71V, I72T, T74A, V82A, I84V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12S+p.I13V+p.K20R+p.L33LF+p.E35D+p.M36I+p.N37E+p.K43IT+p.I54L+p.R57K+p.I62V+p.L63P+p.K70KE+p.A71I+p.V82A+p.I84V+p.L89V+p.L90M+p.Q92K+p.I93L	Missense mutation	22	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12S, I13V, K20R, L33LF, E35D, M36I, N37E, K43IT, I54L, R57K, I62V, L63P, K70KE, A71I, V82A, I84V, L89V, L90M, Q92K, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12TI+p.I13V+p.L19LI+p.K20R+p.L33F+p.E35D+p.M36I+p.N37D+p.K43T+p.M46L+p.I54V+p.K55KR+p.I62V+p.L63P+p.A71IV+p.I72IT+p.G73S+p.V82A+p.L90M+p.I93L	Missense mutation	18	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12TI, I13V, L19LI, K20R, L33F, E35D, M36I, N37D, K43T, M46L, I54V, K55KR, I62V, L63P, A71IV, I72IT, G73S, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.E21D+p.A22V+p.L24M+p.E35DN+p.M36I+p.N37D+p.R41RK+p.M46L+p.I54V+p.R57RK+p.Q61HN+p.I62V+p.L63P+p.I64V+p.I66V+p.A71V+p.I72T+p.G73T+p.N83D+p.I84V	Missense mutation	25	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, E21D, A22V, L24M, E35DN, M36I, N37D, R41RK, M46L, I54V, R57RK, Q61HN, I62V, L63P, I64V, I66V, A71V, I72T, G73T, N83D, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.I13V+p.A22V+p.V32I+p.R41K+p.M46I+p.K55R+p.I62V+p.L63P+p.I64V+p.H69Q+p.A71V+p.V82A+p.I84V	Missense mutation	12	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, I13V, A22V, V32I, R41K, M46I, K55R, I62V, L63P, I64V, H69Q, A71V, V82A, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.I13V+p.G16A+p.V32I+p.L33F+p.M36IL+p.K43T+p.M46L+p.F53L+p.I54L+p.K55R+p.R57G+p.I62V+p.L63P+p.A71V+p.I72R+p.G73S+p.T74P+p.V82I+p.I84V+p.L89V+p.L90M+p.T91S+p.G94S	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, I13V, G16A, V32I, L33F, M36IL, K43T, M46L, F53L, I54L, K55R, R57G, I62V, L63P, A71V, I72R, G73S, T74P, V82I, I84V, L89V, L90M, T91S, G94S.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.I13V+p.K14KE+p.K20R+p.V32I+p.L33F+p.E35D+p.M36I+p.N37S+p.R41K+p.K43T+p.M46I+p.I47V+p.F53L+p.I54L+p.K55R+p.R57G+p.D60E+p.I62V+p.L63P+p.K70KE+p.A71V+p.G73C+p.V82I+p.I84V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, I13V, K14KE, K20R, V32I, L33F, E35D, M36I, N37S, R41K, K43T, M46I, I47V, F53L, I54L, K55R, R57G, D60E, I62V, L63P, K70KE, A71V, G73C, V82I, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.I13V+p.Q18H+p.K20I+p.M46I+p.I54L+p.L63P+p.A71V+p.I72IM+p.G73GS+p.I84V+p.L90M	Missense mutation	14	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, I13V, Q18H, K20I, M46I, I54L, L63P, A71V, I72IM, G73GS, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.T12K+p.I13V+p.K20V+p.V32I+p.L33F+p.E35G+p.M36I+p.N37D+p.M46I+p.I47V+p.I54M+p.R57K+p.Q58E+p.L63P+p.I64V+p.I66V+p.A71V+p.G73S+p.I84V+p.L89V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, T12K, I13V, K20V, V32I, L33F, E35G, M36I, N37D, M46I, I47V, I54M, R57K, Q58E, L63P, I64V, I66V, A71V, G73S, I84V, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.T12K+p.I13V+p.K20V+p.V32I+p.L33LF+p.E35G+p.M36I+p.N37ND+p.M46I+p.I47V+p.I54M+p.R57K+p.Q58E+p.L63P+p.I64V+p.A71IV+p.G73S+p.I84IV+p.L89V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, T12K, I13V, K20V, V32I, L33LF, E35G, M36I, N37ND, M46I, I47V, I54M, R57K, Q58E, L63P, I64V, A71IV, G73S, I84IV, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.T12P+p.I13V+p.K14R+p.V32I+p.L33F+p.N37T+p.K43Q+p.M46I+p.I47V+p.I54L+p.L63P+p.I66F+p.H69Y+p.A71V+p.I72M+p.G73C+p.V77I+p.P79S+p.V82I+p.I84V+p.N88D+p.L89T+p.Q92K	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, T12P, I13V, K14R, V32I, L33F, N37T, K43Q, M46I, I47V, I54L, L63P, I66F, H69Y, A71V, I72M, G73C, V77I, P79S, V82I, I84V, N88D, L89T, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.T12S+p.I13V+p.K14R+p.L19P+p.K20V+p.V32I+p.E34D+p.E35N+p.M36I+p.K43T+p.M46L+p.I47V+p.I54M+p.R57G+p.I62V+p.L63P+p.A71V+p.G73D+p.T74S+p.I84V+p.L89V+p.L90M	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, T12S, I13V, K14R, L19P, K20V, V32I, E34D, E35N, M36I, K43T, M46L, I47V, I54M, R57G, I62V, L63P, A71V, G73D, T74S, I84V, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11L+p.I13V+p.K14R+p.A22V+p.E35D+p.M36I+p.N37D+p.G48M+p.F53L+p.I54V+p.I62V+p.L63P+p.A71V+p.T74S+p.V82A+p.I84V+p.T91S+p.Q92K	Missense mutation	24	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11L, I13V, K14R, A22V, E35D, M36I, N37D, G48M, F53L, I54V, I62V, L63P, A71V, T74S, V82A, I84V, T91S, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11L+p.K20R+p.V32I+p.L33F+p.E34S+p.E35D+p.M36V+p.N37S+p.M46I+p.I47V+p.I54M+p.R57K+p.Q58E+p.I62V+p.L63P+p.A71I+p.V77T+p.V82I+p.I84V+p.I85V+p.L89M+p.L90M+p.I93L	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11L, K20R, V32I, L33F, E34S, E35D, M36V, N37S, M46I, I47V, I54M, R57K, Q58E, I62V, L63P, A71I, V77T, V82I, I84V, I85V, L89M, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11VI+p.I13IV+p.K20R+p.V32VI+p.L33F+p.E35D+p.M36I+p.N37D+p.K43KT+p.M46LS+p.I54L+p.K55R+p.R57K+p.I62V+p.L63T+p.A71V+p.I72V+p.T74PS+p.V82X+p.I84IV+p.L89LF+p.L90M+p.I93L	Missense mutation	22	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11VI, I13IV, K20R, V32VI, L33F, E35D, M36I, N37D, K43KT, M46LS, I54L, K55R, R57K, I62V, L63T, A71V, I72V, T74PS, V82X, I84IV, L89LF, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11VI+p.I13V+p.I15V+p.K20KR+p.V32I+p.L33M+p.N37T+p.K43I+p.I54V+p.D60N+p.I62V+p.L63P+p.I64V+p.A71V+p.T74E+p.V82L+p.I84V+p.Q92R	Missense mutation	51	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11VI, I13V, I15V, K20KR, V32I, L33M, N37T, K43I, I54V, D60N, I62V, L63P, I64V, A71V, T74E, V82L, I84V, Q92R.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11VI+p.I13V+p.K14KR+p.G16A+p.V32I+p.L33F+p.K43T+p.M46L+p.I54L+p.I62V+p.L63P+p.A71V+p.T74P+p.V82VA+p.I84V+p.L90M+p.T91S	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11VI, I13V, K14KR, G16A, V32I, L33F, K43T, M46L, I54L, I62V, L63P, A71V, T74P, V82VA, I84V, L90M, T91S.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11VI+p.T12TP+p.I13IV+p.I15IV+p.K20X+p.L33F+p.E35D+p.M36L+p.N37D+p.K43KT+p.M46L+p.I62V+p.L63P+p.C67F+p.H69HY+p.A71V+p.I72ILM+p.G73S+p.V82A+p.I84V+p.I85V+p.L90M	Missense mutation	15	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11VI, T12TP, I13IV, I15IV, K20X, L33F, E35D, M36L, N37D, K43KT, M46L, I62V, L63P, C67F, H69HY, A71V, I72ILM, G73S, V82A, I84V, I85V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V32I+p.L33F+p.K43Q+p.I47V+p.I54M+p.R57G+p.L63P+p.A71V+p.G73S+p.V77I+p.V82M+p.L90M	Missense mutation	72	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V32I, L33F, K43Q, I47V, I54M, R57G, L63P, A71V, G73S, V77I, V82M, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IT+p.I13V+p.V32I+p.L33F+p.M36I+p.R41RK+p.M46MIL+p.I47A+p.L63P+p.K70E+p.A71V+p.V82A+p.L90M+p.I93L	Missense mutation	16	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IT, I13V, V32I, L33F, M36I, R41RK, M46MIL, I47A, L63P, K70E, A71V, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.I13IV+p.I15V+p.L33F+p.E34Q+p.M36L+p.N37D+p.R41RK+p.M46L+p.G48V+p.I54T+p.I62V+p.L63P+p.A71V+p.I72V+p.T74D+p.V75I+p.V82A+p.L90M+p.I93L	Missense mutation	10	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, I13IV, I15V, L33F, E34Q, M36L, N37D, R41RK, M46L, G48V, I54T, I62V, L63P, A71V, I72V, T74D, V75I, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.V11I+p.G16GE+p.V32I+p.L33F+p.K43R+p.M46L+p.I54M+p.R57K+p.L63P+p.K70T+p.A71V+p.G73S+p.V77I+p.I84V+p.I93L	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, V11I, G16GE, V32I, L33F, K43R, M46L, I54M, R57K, L63P, K70T, A71V, G73S, V77I, I84V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LFI+p.V11VI+p.I13V+p.L33F+p.E35D+p.N37D+p.M46L+p.I54L+p.Q58E+p.I62V+p.L63P+p.A71T+p.I72V+p.L90M+p.Q92R	Missense mutation	12	Potential low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LFI, V11VI, I13V, L33F, E35D, N37D, M46L, I54L, Q58E, I62V, L63P, A71T, I72V, L90M, Q92R.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LFV+p.V11I+p.I13V+p.I15IV+p.K20V+p.L24M+p.V32I+p.L33F+p.E35G+p.M36I+p.I54L+p.K55N+p.Q58QE+p.D60E+p.L63P+p.A71I+p.I72L+p.G73S+p.P79S+p.V82L+p.I84V+p.L89V+p.L90M+p.T91S+p.Q92K	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LFV, V11I, I13V, I15IV, K20V, L24M, V32I, L33F, E35G, M36I, I54L, K55N, Q58QE, D60E, L63P, A71I, I72L, G73S, P79S, V82L, I84V, L89V, L90M, T91S, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LV+p.V11I+p.I13V+p.K20V+p.L24M+p.V32I+p.L33F+p.E35G+p.M36I+p.I54L+p.K55N+p.D60E+p.L63P+p.C67CG+p.A71I+p.I72L+p.G73S+p.P79S+p.I84V+p.L89V+p.L90M+p.T91S+p.Q92K	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LV, V11I, I13V, K20V, L24M, V32I, L33F, E35G, M36I, I54L, K55N, D60E, L63P, C67CG, A71I, I72L, G73S, P79S, I84V, L89V, L90M, T91S, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LV+p.V32I+p.L33M+p.R41K+p.M46I+p.I47V+p.I54M+p.K55KR+p.Q58E+p.L63P+p.K70T+p.A71V+p.T74TP+p.V82T+p.L89F+p.L90M+p.I93L	Missense mutation	36	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LV, V32I, L33M, R41K, M46I, I47V, I54M, K55KR, Q58E, L63P, K70T, A71V, T74TP, V82T, L89F, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.G16A+p.L24I+p.V32I+p.L33F+p.E34EF+p.N37ND+p.M46L+p.I54V+p.L63P+p.A71V+p.I72V+p.V77I+p.V82T+p.I84V	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, G16A, L24I, V32I, L33F, E34EF, N37ND, M46L, I54V, L63P, A71V, I72V, V77I, V82T, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.I15V+p.G16A+p.K20R+p.V32I+p.L33F+p.M36I+p.K43T+p.M46I+p.I47V+p.I54M+p.L63P+p.A71V+p.V82T+p.L90M	Missense mutation	75	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, I15V, G16A, K20R, V32I, L33F, M36I, K43T, M46I, I47V, I54M, L63P, A71V, V82T, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.I15V+p.K20R+p.V32I+p.L33F+p.E35D+p.M36I+p.N37S+p.R41K+p.K43IT+p.M46I+p.I47V+p.F53L+p.R57K+p.L63P+p.A71V+p.G73S+p.V82A+p.L90M+p.I93L+p.C95CF	Missense mutation	44	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, I15V, K20R, V32I, L33F, E35D, M36I, N37S, R41K, K43IT, M46I, I47V, F53L, R57K, L63P, A71V, G73S, V82A, L90M, I93L, C95CF.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.K14R+p.G16E+p.K20R+p.V32I+p.L33F+p.E35D+p.M36I+p.N37E+p.P39PT+p.R41K+p.K43KIT+p.M46I+p.R57K+p.I62V+p.L63AT+p.A71I+p.V77T+p.V82A+p.I84V+p.L90M+p.I93L	Missense mutation	53	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, K14R, G16E, K20R, V32I, L33F, E35D, M36I, N37E, P39PT, R41K, K43KIT, M46I, R57K, I62V, L63AT, A71I, V77T, V82A, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.K20KR+p.V32I+p.L33LI+p.M36IL+p.N37D+p.M46I+p.F53L+p.R57G+p.I62V+p.L63P+p.K70KNT+p.A71I+p.V82A+p.I85IM+p.L89V+p.L90M	Missense mutation	30	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, K20KR, V32I, L33LI, M36IL, N37D, M46I, F53L, R57G, I62V, L63P, K70KNT, A71I, V82A, I85IM, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.K20R+p.V32I+p.E35D+p.M36I+p.K43T+p.M46I+p.I47V+p.F53L+p.I62V+p.L63P+p.A71L+p.I72V+p.V77Q+p.V82C+p.I84V+p.I93L	Missense mutation	23	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, K20R, V32I, E35D, M36I, K43T, M46I, I47V, F53L, I62V, L63P, A71L, I72V, V77Q, V82C, I84V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I15V+p.G16E+p.K20R+p.E35D+p.M36I+p.R41K+p.M46I+p.I50V+p.I54V+p.K55R+p.R57K+p.Q61N+p.I64IL+p.A71V+p.I72R+p.V82A+p.L89I+p.L90M+p.Q92K	Missense mutation	15	Low-level resistance	[3]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I15V, G16E, K20R, E35D, M36I, R41K, M46I, I50V, I54V, K55R, R57K, Q61N, I64IL, A71V, I72R, V82A, L89I, L90M, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.T12X+p.K14KR+p.I15IV+p.Q18QE+p.K20KR+p.V32I+p.L33LF+p.E34EQ+p.E35ED+p.M36I+p.M46I+p.I47A+p.R57K+p.L63P+p.A71V+p.I72V+p.V82A+p.I93L	Missense mutation	18	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, T12X, K14KR, I15IV, Q18QE, K20KR, V32I, L33LF, E34EQ, E35ED, M36I, M46I, I47A, R57K, L63P, A71V, I72V, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.V11I+p.I13V+p.K14R+p.I15V+p.K20T+p.V32I+p.L33F+p.M36I+p.R41K+p.M46L+p.I54L+p.R57K+p.D60E+p.L63P+p.G68E+p.K70T+p.A71I+p.I72M+p.G73S+p.I84V+p.L89V+p.L90M+p.I93L	Missense mutation	100	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, V11I, I13V, K14R, I15V, K20T, V32I, L33F, M36I, R41K, M46L, I54L, R57K, D60E, L63P, G68E, K70T, A71I, I72M, G73S, I84V, L89V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10Y+p.I13V+p.K20R+p.L33F+p.E35D+p.M36L+p.R41K+p.K43KT+p.I62V+p.L63P+p.A71V+p.I72M+p.G73S+p.P79HQ+p.V82L+p.I84V+p.L89V+p.L90M	Missense mutation	22	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10Y, I13V, K20R, L33F, E35D, M36L, R41K, K43KT, I62V, L63P, A71V, I72M, G73S, P79HQ, V82L, I84V, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10Y+p.V11VI+p.I13V+p.Q18QH+p.K20IM+p.E21ED+p.L24LIM+p.L33I+p.M36I+p.R41RK+p.M46I+p.I47IV+p.F53FL+p.I54M+p.I62V+p.L63P+p.I64V+p.I72IM+p.G73V+p.T74PS+p.I84V+p.L89V+p.L90M	Missense mutation	19	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10Y, V11VI, I13V, Q18QH, K20IM, E21ED, L24LIM, L33I, M36I, R41RK, M46I, I47IV, F53FL, I54M, I62V, L63P, I64V, I72IM, G73V, T74PS, I84V, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L5F+p.L10I+p.I13V+p.G16GA+p.K20KR+p.V32I+p.L33F+p.E35ED+p.M36I+p.N37D+p.K43T+p.M46I+p.I47V+p.F53L+p.I54L+p.D60E+p.L63P+p.A71M+p.G73S+p.V82I+p.L90M+p.I93L	Missense mutation	48	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L5F, L10I, I13V, G16GA, K20KR, V32I, L33F, E35ED, M36I, N37D, K43T, M46I, I47V, F53L, I54L, D60E, L63P, A71M, G73S, V82I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T4TS+p.L10F+p.V11I+p.T12P+p.I13V+p.I15IV+p.K20IT+p.L24M+p.E35D+p.M36I+p.M46I+p.F53FL+p.Q58QE+p.I62V+p.L63E+p.C67CY+p.A71I+p.I72T+p.G73S+p.T74P+p.P79AG+p.I84AV+p.L89V+p.L90M+p.Q92K+p.I93L	Missense mutation	36	Intermediate resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T4TS, L10F, V11I, T12P, I13V, I15IV, K20IT, L24M, E35D, M36I, M46I, F53FL, Q58QE, I62V, L63E, C67CY, A71I, I72T, G73S, T74P, P79AG, I84AV, L89V, L90M, Q92K, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V11VI+p.I15IV+p.K20I+p.E34Q+p.M46I+p.I47V+p.F53FL+p.I54M+p.R57G+p.D60E+p.Q61H+p.L63P+p.A71V+p.G73T+p.V77I+p.P79DN+p.I84V+p.I85V+p.L90M+p.I93L	Missense mutation	25	Low-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V11VI, I15IV, K20I, E34Q, M46I, I47V, F53FL, I54M, R57G, D60E, Q61H, L63P, A71V, G73T, V77I, P79DN, I84V, I85V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Resistant Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V32I+p.L33F+p.E34Q+p.M36A+p.P39S+p.M46I+p.F53L+p.I54L+p.K55KR+p.I62V+p.L63P+p.A71V+p.I84V+p.L90M	Missense mutation	94	High-level resistance	[1]

Resistant Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V32I, L33F, E34Q, M36A, P39S, M46I, F53L, I54L, K55KR, I62V, L63P, A71V, I84V, L90M.

Drug Sensitivity Data Categorized by Their Corresponding Mechanisms

Aberration of the Drug's Therapeutic Target (ADTT)

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Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.G16E+p.M36MI+p.P39S+p.R41K+p.R57G+p.L63T+p.A71V+p.I93L	Missense mutation	0.8	Susceptible	[4]
p.R57G+p.L63AT+p.E65D+p.I72V+p.V77I	Missense mutation	0.6	Susceptible	[4]
p.K14R+p.Q18E+p.E35D+p.M36I+p.R57G+p.I62V+p.L63P+p.I72V+p.I93L	Missense mutation	0.5	Susceptible	[4]
p.I13V+p.R57G+p.L63D+p.I64V+p.E65D+p.K70R+p.I72T	Missense mutation	0.6	Susceptible	[4]
p.T12TA+p.I13V+p.G17E+p.K20I+p.M36I+p.R41K+p.R57G+p.L63P+p.H69K+p.K70KR+p.I72L+p.L89I	Missense mutation	0.6	Susceptible	[4]
p.T12TP+p.I13IV+p.K14KR+p.N37S+p.R57K+p.L63P+p.I64V+p.C67X	Missense mutation	0.9	Susceptible	[4]
p.E35D+p.N37S+p.R57K+p.I62IV+p.L63P	Missense mutation	0.8	Susceptible	[4]
p.N37T+p.R57K+p.I62V+p.L63ST+p.A71T+p.I72V+p.V77I+p.I93L	Missense mutation	0.6	Susceptible	[4]
p.E35D+p.M36I+p.N37D+p.R57K+p.D60DE+p.I62V+p.L63P+p.V77I+p.I93L	Missense mutation	0.8	Susceptible	[4]
p.I13V+p.L19I+p.K20T+p.E35G+p.M36I+p.R41K+p.R57G+p.I62V+p.L63T+p.I64V+p.I72T+p.T74S+p.V77I+p.L90M	Missense mutation	2.9	Susceptible	[4]
p.T12PS+p.I13V+p.L19LI+p.N37A+p.R57G+p.L63P+p.I64V	Missense mutation	0.9	Susceptible	[4]
p.E35D+p.N37S+p.R41K+p.R57G+p.I62IV+p.L63A+p.I72IV	Missense mutation	0.8	Susceptible	[4]
p.R57G+p.L63P+p.I72E+p.V77I+p.I93L	Missense mutation	0.6	Susceptible	[4]
p.I13V+p.I15L+p.L33V+p.N37S+p.R41K+p.K45KR+p.R57K+p.L63X+p.I64V	Missense mutation	0.7	Susceptible	[4]
p.T12S+p.L19I+p.E35D+p.R57K+p.L63P+p.H69Q+p.A71AT	Missense mutation	0.9	Susceptible	[4]
p.L10LF+p.K14KR+p.E35D+p.R41RK+p.R57RG+p.L63P	Missense mutation	1.1	Susceptible	[4]
p.T12P+p.N37S+p.R57G+p.L63A+p.I72V+p.V77I+p.I93L	Missense mutation	0.6	Susceptible	[4]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

GeneSeq assay; PhenoSense GT assay

Experiment for
Drug Resistance

Viral load assay

Mechanism Description

Amino acid changes in IN may contribute to drug resistance or sensitivity.

Key Molecule: HIV2 Protease (HIV2 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I32V+p.V47I+p.M76L+p.I82V	Missense mutation	0	Susceptible	[5]
p.I82V	Missense mutation	0.5	Susceptible	[5]
p.M76L	Missense mutation	0.5	Susceptible	[5]
p.V47I	Missense mutation	0.3	Susceptible	[5]
p.I32V	Missense mutation	0.2	Susceptible	[5]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

In Vitro Model

HEK293T/17 cells

Kidney

Homo sapiens (Human)

CVCL_1926

Experiment for
Molecule Alteration

Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; A single-cycle assay

Mechanism Description

Single amino acid replacements I32V, V47I, and M76L increased the susceptibility of HIV-2 to multiple PI, but no single change conferred class-wide sensitivity. In contrast, clone PRdeta4 showed PI susceptibility equivalent to or greater than that of HIV-1 for all PI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.A22AV+p.R41K+p.R57G+p.D60E+p.L63P+p.V77I+p.Q92E	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included A22AV, R41K, R57G, D60E, L63P, V77I, Q92E.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.D30N+p.L33F+p.I62V+p.L63P+p.I64L+p.A71AV+p.V77I+p.N88D+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included D30N, L33F, I62V, L63P, I64L, A71AV, V77I, N88D, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E34Q+p.N37C+p.R41K+p.M46I+p.I50V+p.I62V+p.L63T+p.A71V+p.L76V+p.V77I+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E34Q, N37C, R41K, M46I, I50V, I62V, L63T, A71V, L76V, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.K43KR+p.R57G+p.L63P+p.V82VI+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, K43KR, R57G, L63P, V82VI, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.L63P	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.M36I+p.I62IV+p.L63P+p.A71AV+p.G73GS+p.V77VI+p.I84IV+p.L90LM	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, M36I, I62IV, L63P, A71AV, G73GS, V77VI, I84IV, L90LM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.M36I+p.L63P	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, M36I, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.M36I+p.N37D+p.R41RK+p.I64V+p.H69Y	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, M36I, N37D, R41RK, I64V, H69Y.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.M36I+p.N37ND+p.R41K+p.R57RK+p.I62IV+p.L63P+p.A71V+p.L90M+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, M36I, N37ND, R41K, R57RK, I62IV, L63P, A71V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.M36I+p.R41K+p.I62V+p.L63P+p.V77I+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, M36I, R41K, I62V, L63P, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.M36I+p.R57G+p.I62IV+p.L63P+p.I64L	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, M36I, R57G, I62IV, L63P, I64L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.M36I+p.R57G+p.L63P+p.I64L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, M36I, R57G, L63P, I64L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.M36MI+p.L63P	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, M36MI, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.N37D+p.L63P+p.A71T+p.I93L	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, N37D, L63P, A71T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.N37D+p.R57G+p.I62V+p.L63P+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, N37D, R57G, I62V, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.N37D+p.R57G+p.L63P+p.A71AV	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, N37D, R57G, L63P, A71AV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.N37D+p.R57G+p.L63P+p.I64IV	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, N37D, R57G, L63P, I64IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.N37D+p.R57K+p.L63P+p.A71T+p.I72IV+p.V77I+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, N37D, R57K, L63P, A71T, I72IV, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.N37D+p.R57K+p.L63P+p.A71T+p.I72V+p.V77I+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, N37D, R57K, L63P, A71T, I72V, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.N37Y+p.R41K+p.R57K+p.L63LP+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, N37Y, R41K, R57K, L63LP, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.R41K+p.L63P+p.I64L+p.A71AV+p.I84IV	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, R41K, L63P, I64L, A71AV, I84IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.R41RK+p.R57G+p.Q61H+p.I64IV+p.V77L+p.I93L	Missense mutation	0.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, R41RK, R57G, Q61H, I64IV, V77L, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.R57G+p.L63P+p.H69Q	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, R57G, L63P, H69Q.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.R57G+p.L63P+p.I64V+p.I72IM	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, R57G, L63P, I64V, I72IM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.R57G+p.L63P+p.I72V	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, R57G, L63P, I72V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35D+p.R57RK+p.L63P+p.I64IV	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35D, R57RK, L63P, I64IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.E35X+p.N37X+p.L38LM+p.R41RK+p.R57G+p.I62IV+p.L63HP+p.A71AV+p.V77I+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included E35X, N37X, L38LM, R41RK, R57G, I62IV, L63HP, A71AV, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.G16E+p.K55KR+p.R57G+p.I72IR+p.V77VI	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included G16E, K55KR, R57G, I72IR, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.G16E+p.N37S+p.I62V+p.L63T+p.I72V+p.V77I	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included G16E, N37S, I62V, L63T, I72V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.G17E+p.D60E+p.L63P+p.I64V+p.K70R+p.V77VI	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included G17E, D60E, L63P, I64V, K70R, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13A+p.K14R+p.K20I+p.M36I+p.N37D+p.R41K+p.H69K+p.L89I	Missense mutation	1	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13A, K14R, K20I, M36I, N37D, R41K, H69K, L89I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IA+p.I15IV+p.N37NS+p.R57G+p.I72IV	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IA, I15IV, N37NS, R57G, I72IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IM+p.I15IV+p.L19LI+p.R41K+p.Q61QR+p.L63LP+p.I72V+p.V77I+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IM, I15IV, L19LI, R41K, Q61QR, L63LP, I72V, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.G16GE+p.L19V+p.K20I+p.L63P+p.K70R+p.A71T	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, G16GE, L19V, K20I, L63P, K70R, A71T.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.I15IV+p.K20X+p.D60E+p.Q61E+p.L63P+p.I64M	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, I15IV, K20X, D60E, Q61E, L63P, I64M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.I15IV+p.M36MI+p.R57G+p.I72IV	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, I15IV, M36MI, R57G, I72IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.K14KR+p.E35D+p.M36I+p.N37ND+p.R41K+p.D60DE+p.Q61QE+p.I62IV+p.L63X+p.I64ILM+p.C67CY+p.H69K+p.L89M+p.I93IL	Missense mutation	0.8	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, K14KR, E35D, M36I, N37ND, R41K, D60DE, Q61QE, I62IV, L63X, I64ILM, C67CY, H69K, L89M, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.K14KR+p.N37CS+p.R41RK+p.I64V+p.V77I	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, K14KR, N37CS, R41RK, I64V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.K20KIM+p.M36MI+p.N37D+p.R41K+p.D60E+p.I62V+p.L63P+p.I64IL+p.H69Y+p.I72E	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, K20KIM, M36MI, N37D, R41K, D60E, I62V, L63P, I64IL, H69Y, I72E.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.M36I+p.N37D+p.R57G+p.L63AS	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, M36I, N37D, R57G, L63AS.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.N37NS+p.R41K+p.L63A+p.I64V+p.H69Y+p.V77I	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, N37NS, R41K, L63A, I64V, H69Y, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.N37NT+p.R57K+p.Q58QE+p.D60E+p.Q61QE+p.L63P+p.I64IL+p.V77VI+p.N88NS	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, N37NT, R57K, Q58QE, D60E, Q61QE, L63P, I64IL, V77VI, N88NS.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.N37T+p.I62V+p.L63HQ+p.I72IV	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, N37T, I62V, L63HQ, I72IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.R41K+p.K55R+p.R57G+p.L63H+p.I64V+p.E65D+p.V77I	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, R41K, K55R, R57G, L63H, I64V, E65D, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.D30N+p.K43T+p.M46I+p.D60E+p.Q61E+p.L63P+p.V77I+p.I93L	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, D30N, K43T, M46I, D60E, Q61E, L63P, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.E35D+p.N37T+p.L63P+p.I64V	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, E35D, N37T, L63P, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.I15IV+p.Q18QH+p.R57RK+p.I62IV+p.L63P+p.I64IV+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, I15IV, Q18QH, R57RK, I62IV, L63P, I64IV, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.I15V+p.K20R+p.M36I+p.R41K+p.H69K+p.L89M+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, I15V, K20R, M36I, R41K, H69K, L89M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.I15V+p.K20R+p.N37NS+p.L63P+p.V77I	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, I15V, K20R, N37NS, L63P, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.I15V+p.K20R+p.N37S+p.L63P+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, I15V, K20R, N37S, L63P, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.I15V+p.K20T+p.E35D+p.M36I+p.R41K+p.I62V+p.L63P+p.H69K+p.A71T+p.T74S+p.L89I	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, I15V, K20T, E35D, M36I, R41K, I62V, L63P, H69K, A71T, T74S, L89I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.I15V+p.P39Q+p.R57K+p.L63P+p.I64V	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, I15V, P39Q, R57K, L63P, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.I64V+p.I72V	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, I64V, I72V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.I64V	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14KR+p.G16E+p.K20R+p.E35D+p.M36I+p.N37X+p.R41K+p.L63LIV+p.H69K+p.L89M	Missense mutation	0.7	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14KR, G16E, K20R, E35D, M36I, N37X, R41K, L63LIV, H69K, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14KR+p.I15IV+p.K20I+p.M36I+p.R41K+p.I64M+p.H69K+p.K70R+p.V82I+p.L89M	Missense mutation	1.3	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14KR, I15IV, K20I, M36I, R41K, I64M, H69K, K70R, V82I, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14KR+p.I15V+p.L19I+p.M36I+p.K43R+p.R57K+p.L63P+p.L89M	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14KR, I15V, L19I, M36I, K43R, R57K, L63P, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.E35D+p.M36I+p.R41K+p.R57K+p.H69K+p.L89M	Missense mutation	1.4	Susceptible	[7]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, E35D, M36I, R41K, R57K, H69K, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.G16E+p.K20I+p.E35D+p.M36I+p.R41K+p.M46ML+p.H69K+p.L89M	Missense mutation	0.7	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, G16E, K20I, E35D, M36I, R41K, M46ML, H69K, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.G16E+p.K20R+p.E35D+p.M36I+p.P39PS+p.R41K+p.H69K+p.L89M+p.I93L	Missense mutation	0.7	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, G16E, K20R, E35D, M36I, P39PS, R41K, H69K, L89M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.I15V+p.G16A+p.E35D+p.N37D+p.L63P	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, I15V, G16A, E35D, N37D, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.I15V+p.Q18K+p.K20T+p.V32I+p.E35D+p.M36I+p.N37D+p.R57K+p.L63P+p.I64V+p.A71T+p.I72IV+p.V77I+p.V82I+p.L90M+p.I93L	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, I15V, Q18K, K20T, V32I, E35D, M36I, N37D, R57K, L63P, I64V, A71T, I72IV, V77I, V82I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.I15V+p.Q18QK+p.K20RT+p.V32I+p.E35D+p.M36I+p.N37D+p.R57K+p.L63P+p.I64IV+p.A71IMT+p.I72V+p.V77I+p.V82VI+p.L90M+p.I93L	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, I15V, Q18QK, K20RT, V32I, E35D, M36I, N37D, R57K, L63P, I64IV, A71IMT, I72V, V77I, V82VI, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.K20I+p.E35D+p.M36I+p.R41K+p.H69K+p.V82I+p.L89M	Missense mutation	1	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, K20I, E35D, M36I, R41K, H69K, V82I, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.K20I+p.E35D+p.M36I+p.R41K+p.I62IV+p.L63LHP+p.H69K+p.V82I+p.L89M	Missense mutation	1.2	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, K20I, E35D, M36I, R41K, I62IV, L63LHP, H69K, V82I, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.K20I+p.E35D+p.M36I+p.R41K+p.I64L+p.H69K+p.V82VI+p.L89M	Missense mutation	0.6	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, K20I, E35D, M36I, R41K, I64L, H69K, V82VI, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.K20I+p.M36I+p.R41K+p.I64M+p.H69K+p.K70R+p.V82I+p.L89M	Missense mutation	1	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, K20I, M36I, R41K, I64M, H69K, K70R, V82I, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.K20I+p.V32VA+p.M36I+p.R41K+p.H69K+p.L89M	Missense mutation	0.7	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, K20I, V32VA, M36I, R41K, H69K, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.K20IV+p.M36I+p.N37ND+p.R41K+p.L63S+p.H69K+p.L89M	Missense mutation	0.9	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, K20IV, M36I, N37ND, R41K, L63S, H69K, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.K20KIM+p.R41RK+p.I64V+p.V77IL	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, K20KIM, R41RK, I64V, V77IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.L19I+p.K20I+p.E35Q+p.M36I+p.R41K+p.R57K+p.C67E+p.H69K+p.V82I+p.L89M	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, L19I, K20I, E35Q, M36I, R41K, R57K, C67E, H69K, V82I, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K14R+p.L19P+p.K20I+p.E35D+p.M36I+p.R41K+p.H69K+p.V82I+p.L89M	Missense mutation	1.4	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K14R, L19P, K20I, E35D, M36I, R41K, H69K, V82I, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K20I+p.E35DN+p.M36V+p.I62V+p.L63P+p.T74TS+p.V75VI+p.L90M+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K20I, E35DN, M36V, I62V, L63P, T74TS, V75VI, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K45R+p.I62IV+p.L63S+p.I64M+p.V77I	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K45R, I62IV, L63S, I64M, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.L19I+p.E35D+p.N37X+p.L63P	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, L19I, E35D, N37X, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.L19V+p.M36MI+p.R41K+p.Q61N+p.I64V+p.E65D+p.I72E	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, L19V, M36MI, R41K, Q61N, I64V, E65D, I72E.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.L33LF+p.M36ML+p.N37ND+p.K45KN+p.I50IL+p.I54IV+p.L63LP+p.I64V+p.I72IT+p.V82VA	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, L33LF, M36ML, N37ND, K45KN, I50IL, I54IV, L63LP, I64V, I72IT, V82VA.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.L33LV+p.R41RK+p.L63LP+p.I64IV+p.I72IM	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, L33LV, R41RK, L63LP, I64IV, I72IM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.M36I+p.R57G+p.L63P+p.A71T+p.V77I+p.I93L	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, M36I, R57G, L63P, A71T, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.M46MI+p.L63P+p.I64V+p.K70R+p.I72V+p.V77VI+p.L90M+p.I93IL	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, M46MI, L63P, I64V, K70R, I72V, V77VI, L90M, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.N37D+p.R57G+p.L63S+p.H69R	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, N37D, R57G, L63S, H69R.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.N37NS+p.L63P+p.I72E	Missense mutation	0.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, N37NS, L63P, I72E.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.N37ST+p.I62IV+p.L63P+p.K70R+p.A71AT+p.I72IM	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, N37ST, I62IV, L63P, K70R, A71AT, I72IM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.N37T+p.L63E+p.I64V+p.E65D+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, N37T, L63E, I64V, E65D, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.N37T+p.L63P+p.T74TA	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, N37T, L63P, T74TA.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.P39PT+p.R57G+p.L63P+p.V77VI	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, P39PT, R57G, L63P, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.P39Q+p.I62V+p.L63P+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, P39Q, I62V, L63P, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.Q18H+p.L63P	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, Q18H, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.R41K+p.D60E+p.L63P	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, R41K, D60E, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.R41K+p.L63P+p.H69R+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, R41K, L63P, H69R, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.R57G+p.I62IV+p.L63P	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, R57G, I62IV, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.R57G+p.L63P+p.K70R+p.I72IV+p.V77VI	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, R57G, L63P, K70R, I72IV, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.R57G+p.L63P	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, R57G, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15IV+p.I64IV	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15IV, I64IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15IV+p.K43KR+p.I62V+p.L63P+p.I72IV+p.V77I+p.L90LM+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15IV, K43KR, I62V, L63P, I72IV, V77I, L90LM, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15IV+p.M36I+p.R41K+p.R57RK+p.L63LP+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15IV, M36I, R41K, R57RK, L63LP, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15IV+p.N37X+p.L38IM+p.R41RK+p.R57G+p.I62V+p.I64IV+p.H69HY+p.I72IMV+p.V77VI	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15IV, N37X, L38IM, R41RK, R57G, I62V, I64IV, H69HY, I72IMV, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15IV+p.Q18H+p.E21EG+p.N37S+p.K43KR+p.R57K+p.L63P+p.V77VI	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15IV, Q18H, E21EG, N37S, K43KR, R57K, L63P, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15IV+p.R41K+p.R57K+p.L63P+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15IV, R41K, R57K, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.E35D+p.L63P+p.A71AV+p.I72IT+p.V82L+p.I93L	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, E35D, L63P, A71AV, I72IT, V82L, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.E35D+p.M36T+p.N37E+p.R57G+p.I62V+p.L63P	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, E35D, M36T, N37E, R57G, I62V, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.E35D+p.N37S+p.R57G+p.L63P+p.I72V+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, E35D, N37S, R57G, L63P, I72V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.E35D+p.P39PS+p.R57G	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, E35D, P39PS, R57G.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.E35D+p.R57G+p.L63LP	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, E35D, R57G, L63LP.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.E35D+p.R57G+p.L63P	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, E35D, R57G, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.E35D+p.R57G	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, E35D, R57G.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.E35ED+p.M36MI+p.N37X+p.R41RK+p.I62IL+p.L63LP+p.A71AV+p.V77I+p.N83ND+p.C95CR	Missense mutation	0.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, E35ED, M36MI, N37X, R41RK, I62IL, L63LP, A71AV, V77I, N83ND, C95CR.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.E35ED+p.N37C+p.R41RK+p.L63LP+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, E35ED, N37C, R41RK, L63LP, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.G16E+p.D60E+p.Q61QE+p.I62V+p.L63KQ+p.I72EV+p.V77I	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, G16E, D60E, Q61QE, I62V, L63KQ, I72EV, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.I62V+p.L63P	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, I62V, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.K20KR+p.M36I+p.R57RK+p.I62V+p.L63P+p.I72IV+p.V82L+p.I93L	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, K20KR, M36I, R57RK, I62V, L63P, I72IV, V82L, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.K20R+p.E35D+p.M36I+p.N37D+p.R41K+p.R57K+p.V82I+p.I93L	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, K20R, E35D, M36I, N37D, R41K, R57K, V82I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.K20X+p.M36I+p.R41RK+p.I62V+p.L63P+p.A71T+p.I72T+p.L90M	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, K20X, M36I, R41RK, I62V, L63P, A71T, I72T, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.L19IT+p.M36MIL+p.I62V	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, L19IT, M36MIL, I62V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.L19T+p.K20R+p.M36I+p.R41K+p.I62V+p.K70R+p.V82L	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, L19T, K20R, M36I, R41K, I62V, K70R, V82L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.L19T+p.M36I+p.R41K+p.L63Q+p.H69K+p.I72IM+p.L89LM+p.L90LS+p.I93L	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, L19T, M36I, R41K, L63Q, H69K, I72IM, L89LM, L90LS, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.L63A+p.V77I+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, L63A, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.M36I+p.I64V+p.V82L	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, M36I, I64V, V82L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.M36I+p.I72V	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, M36I, I72V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.M36I+p.L63C	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, M36I, L63C.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.M36I+p.N37D+p.R57G+p.L63P+p.I93L	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, M36I, N37D, R57G, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.M36I+p.N37S+p.R41K+p.L63P+p.H69K+p.L89M+p.I93L	Missense mutation	0.9	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, M36I, N37S, R41K, L63P, H69K, L89M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.M36I+p.R41K	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, M36I, R41K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.M36MI+p.N37D+p.L63X+p.H69Y	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, M36MI, N37D, L63X, H69Y.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.M36MI+p.N37D+p.R57G+p.L63X+p.H69Y	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, M36MI, N37D, R57G, L63X, H69Y.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.N37D+p.K70R+p.V77I+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, N37D, K70R, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.N37S+p.R41K+p.I64IV	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, N37S, R41K, I64IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.N37T+p.R41K+p.K43KR+p.L63Q	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, N37T, R41K, K43KR, L63Q.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.R41K+p.L63A+p.I64V+p.K70R	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, R41K, L63A, I64V, K70R.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.R57G+p.I62V+p.L63Q+p.V77VI+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, R57G, I62V, L63Q, V77VI, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I3IV+p.I13IV+p.I64V+p.C67CG+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I3IV, I13IV, I64V, C67CG, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I3L+p.L10I+p.R41K+p.R57K+p.L63Q+p.I64V+p.I72V+p.V77I	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I3L, L10I, R41K, R57K, L63Q, I64V, I72V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I3M+p.K14R+p.R41K+p.R57G+p.L63P+p.V77I	Missense mutation	0.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I3M, K14R, R41K, R57G, L63P, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I64V+p.V77I+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I64V, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I64V	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14E+p.L19I+p.L33V+p.N37C+p.R41K+p.K45KR+p.I62V+p.I64V+p.E65K+p.H69Y+p.A71AV+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14E, L19I, L33V, N37C, R41K, K45KR, I62V, I64V, E65K, H69Y, A71AV, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14KR+p.E35D+p.N37NS+p.R41K	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14KR, E35D, N37NS, R41K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14KR+p.I15IV+p.L19T+p.R41K+p.I64IV	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14KR, I15IV, L19T, R41K, I64IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14KR+p.L19I+p.D60DE+p.L63X+p.H69HY+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14KR, L19I, D60DE, L63X, H69HY, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14KR+p.R57K+p.L63P+p.V77I+p.I84IV+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14KR, R57K, L63P, V77I, I84IV, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14R+p.E35D+p.M36I+p.N37E+p.R57G+p.L63P+p.A71V+p.V77I+p.L90M+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14R, E35D, M36I, N37E, R57G, L63P, A71V, V77I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14R+p.I15V+p.G16E+p.K20KT+p.M36I+p.P39S+p.D60E+p.I62V+p.L63P+p.I72V	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14R, I15V, G16E, K20KT, M36I, P39S, D60E, I62V, L63P, I72V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14R+p.I15V+p.R41K+p.L63P	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14R, I15V, R41K, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14R+p.I15V+p.R41K+p.R57G+p.L63Q+p.A71V+p.I93L	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14R, I15V, R41K, R57G, L63Q, A71V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14R+p.L19I+p.E35D+p.N37D+p.D60E+p.I62V+p.L63P+p.V77I+p.I93L	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14R, L19I, E35D, N37D, D60E, I62V, L63P, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14R+p.L33V+p.R41K+p.I64V	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14R, L33V, R41K, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14R+p.N37D+p.R41K+p.I62V+p.L63A+p.V77I+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14R, N37D, R41K, I62V, L63A, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14R+p.N37S+p.R41K+p.I64V+p.Q92K	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14R, N37S, R41K, I64V, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K14R+p.R57G+p.L63P+p.I64L+p.I72V+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K14R, R57G, L63P, I64L, I72V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KI+p.L63A+p.A71AV+p.V77I+p.L90LM+p.I93IL	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KI, L63A, A71AV, V77I, L90LM, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.E35D+p.M36I+p.R57RK+p.I62V+p.L63APS+p.L90M	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, E35D, M36I, R57RK, I62V, L63APS, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.E35ED+p.M36I+p.N37D+p.R41RK+p.L63P+p.I64L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, E35ED, M36I, N37D, R41RK, L63P, I64L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E35D+p.M36I+p.R41K+p.K43KR+p.R57K+p.D60E+p.I62V+p.L63P+p.A71V+p.I72T+p.P79D+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E35D, M36I, R41K, K43KR, R57K, D60E, I62V, L63P, A71V, I72T, P79D, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20R+p.E35D+p.P39PS+p.R57G+p.L63LP+p.A71V+p.I72IV+p.V77VIM+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20R, E35D, P39PS, R57G, L63LP, A71V, I72IV, V77VIM, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20V+p.F53L+p.L63P+p.A71V+p.G73S+p.V77I+p.I85IV+p.L90M	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20V, F53L, L63P, A71V, G73S, V77I, I85IV, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K43KT+p.I54V+p.L63P+p.A71V+p.I72V+p.V82A	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K43KT, I54V, L63P, A71V, I72V, V82A.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K45R+p.I50L+p.I62V+p.L63P+p.A71IT+p.I72V+p.G73GS+p.V77I+p.V82A+p.I93L	Missense mutation	0.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K45R, I50L, I62V, L63P, A71IT, I72V, G73GS, V77I, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.E34ED+p.M36MI+p.M46MI+p.I54IL+p.K55R+p.R57K+p.Q58QE+p.I62V+p.L63LP+p.A71T+p.N83NS+p.I84V+p.L89X	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, E34ED, M36MI, M46MI, I54IL, K55R, R57K, Q58QE, I62V, L63LP, A71T, N83NS, I84V, L89X.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.G16GE+p.L24LI+p.D30N+p.L33I+p.E35D+p.M36I+p.N37S+p.K45Q+p.M46MI+p.I54V+p.R57G+p.D60E+p.I62V+p.L63S+p.I64V+p.I72IM+p.Q92QK	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, G16GE, L24LI, D30N, L33I, E35D, M36I, N37S, K45Q, M46MI, I54V, R57G, D60E, I62V, L63S, I64V, I72IM, Q92QK.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.K20R+p.D30N+p.L33I+p.E35D+p.M36I+p.M46L+p.I54V+p.R57K+p.L63P+p.A71V+p.V82A+p.N88D+p.L90M+p.I93L	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, K20R, D30N, L33I, E35D, M36I, M46L, I54V, R57K, L63P, A71V, V82A, N88D, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.L19LQ+p.K20I+p.E34Q+p.E35D+p.M36V+p.N37E+p.M46I+p.F53L+p.I54V+p.R57K+p.L63P+p.A71T+p.V77I+p.V82A+p.L90M+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, L19LQ, K20I, E34Q, E35D, M36V, N37E, M46I, F53L, I54V, R57K, L63P, A71T, V77I, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I15V+p.R41K+p.M46I+p.K55R+p.I62IV+p.L63P+p.A71T+p.G73GS+p.V82T+p.I84V+p.I85V+p.L89LM	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I15V, R41K, M46I, K55R, I62IV, L63P, A71T, G73GS, V82T, I84V, I85V, L89LM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.N37A+p.R41K+p.R57G+p.L63T+p.E65D+p.I72T+p.V77I	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, N37A, R41K, R57G, L63T, E65D, I72T, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.T12K+p.I13V+p.L19MT+p.K20I+p.M36I+p.R41K+p.M46I+p.I47IV+p.I54V+p.K55R+p.Q58E+p.I62V+p.L63S+p.I64V+p.E65D+p.V82VA	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, T12K, I13V, L19MT, K20I, M36I, R41K, M46I, I47IV, I54V, K55R, Q58E, I62V, L63S, I64V, E65D, V82VA.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.T12TN+p.K14KR+p.L24I+p.L33F+p.M46I+p.I54V+p.I62V+p.L63P+p.H69Q+p.A71I+p.I72L+p.V77I+p.V82T+p.I93M	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, T12TN, K14KR, L24I, L33F, M46I, I54V, I62V, L63P, H69Q, A71I, I72L, V77I, V82T, I93M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.T12TP+p.I13V+p.K14KR+p.K20I+p.E35D+p.M36I+p.N37D+p.M46I+p.I54V+p.L63P+p.A71V+p.I72V+p.V82A+p.L90M+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, T12TP, I13V, K14KR, K20I, E35D, M36I, N37D, M46I, I54V, L63P, A71V, I72V, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10FIV+p.I13IV+p.D30N+p.E35D+p.M36IV+p.N37X+p.R41RK+p.Q58E+p.I62V+p.L63P+p.I64V+p.N88D+p.I93IL	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10FIV, I13IV, D30N, E35D, M36IV, N37X, R41RK, Q58E, I62V, L63P, I64V, N88D, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10H+p.I13IV+p.Q18H+p.L19IM+p.K20T+p.R41K+p.M46I+p.D60E+p.L63P+p.A71I+p.V77I+p.I93L+p.C95CY	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10H, I13IV, Q18H, L19IM, K20T, R41K, M46I, D60E, L63P, A71I, V77I, I93L, C95CY.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.E35D+p.M36I+p.R41K+p.R57G+p.I62V+p.L63P+p.I64V+p.I85V+p.L90M+p.I93L	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, E35D, M36I, R41K, R57G, I62V, L63P, I64V, I85V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.E35D+p.P39PQS+p.L63P+p.A71AT+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, E35D, P39PQS, L63P, A71AT, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.G16E+p.L63PT+p.T74P+p.I93L	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, G16E, L63PT, T74P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.D30N+p.R41K+p.L63P+p.I64V+p.V75I+p.V77I+p.N88D+p.L90M+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, D30N, R41K, L63P, I64V, V75I, V77I, N88D, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.D60E+p.I62V+p.L63A+p.I64L+p.A71T+p.I72V+p.V77I	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, D60E, I62V, L63A, I64L, A71T, I72V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K14R+p.I15V+p.K20I+p.E35Q+p.M36I+p.R41K+p.R57K+p.C67E+p.H69K+p.V82I+p.L89M	Missense mutation	1.4	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K14R, I15V, K20I, E35Q, M36I, R41K, R57K, C67E, H69K, V82I, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K14R+p.K20I+p.M36I+p.N37S+p.R41K+p.I64L+p.H69K+p.K70R+p.L89M	Missense mutation	0.6	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K14R, K20I, M36I, N37S, R41K, I64L, H69K, K70R, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20I+p.E35Q+p.M36I+p.N37D+p.R41K+p.K43R+p.R57K+p.C67E+p.H69K+p.V82I+p.L89M	Missense mutation	1.1	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20I, E35Q, M36I, N37D, R41K, K43R, R57K, C67E, H69K, V82I, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20I+p.L33V+p.M46L+p.L63P+p.A71T+p.I72V+p.T74K+p.I85V+p.N88D+p.L90M+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20I, L33V, M46L, L63P, A71T, I72V, T74K, I85V, N88D, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20L+p.M46I+p.Q58E+p.I62V+p.L63P+p.A71T+p.T74S+p.V77I+p.N88S+p.L90M+p.Q92K	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20L, M46I, Q58E, I62V, L63P, A71T, T74S, V77I, N88S, L90M, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20R+p.E35D+p.M36I+p.I62V+p.L63P+p.I64L+p.T74S+p.L90M	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20R, E35D, M36I, I62V, L63P, I64L, T74S, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20T+p.D60E+p.L63P+p.C67D+p.A71V+p.V77I+p.L90M+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20T, D60E, L63P, C67D, A71V, V77I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L19V+p.K20T+p.M46I+p.I54V+p.I62V+p.L63P+p.A71V+p.I72L+p.G73S+p.V82T+p.L90M	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L19V, K20T, M46I, I54V, I62V, L63P, A71V, I72L, G73S, V82T, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L24I+p.L33F+p.M36L+p.K43T+p.M46I+p.I54V+p.D60E+p.I62V+p.L63P+p.A71V+p.T74S+p.V82A+p.N83D+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L24I, L33F, M36L, K43T, M46I, I54V, D60E, I62V, L63P, A71V, T74S, V82A, N83D, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L63P+p.H69Q+p.I72V+p.V77I	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L63P, H69Q, I72V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.R57G+p.D60E+p.I62IV+p.L63A+p.I64ILM+p.A71AT+p.I72IV+p.V77I	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, R57G, D60E, I62IV, L63A, I64ILM, A71AT, I72IV, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.R57G+p.I64V+p.V77VI	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, R57G, I64V, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.R57G+p.I64V	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, R57G, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15IL+p.K43KR+p.L63P+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15IL, K43KR, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15IV+p.L23LI+p.D29DG+p.I50IL+p.K55KR+p.L63P+p.I72E+p.G73GS+p.I84IV+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15IV, L23LI, D29DG, I50IL, K55KR, L63P, I72E, G73GS, I84IV, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.L19I+p.K20M+p.L24I+p.E35D+p.M36I+p.G48Q+p.F53L+p.I54V+p.L63P+p.K70E+p.A71V+p.I72M+p.G73S+p.V75VI+p.V82A+p.I93IM	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, L19I, K20M, L24I, E35D, M36I, G48Q, F53L, I54V, L63P, K70E, A71V, I72M, G73S, V75VI, V82A, I93IM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.L63A+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, L63A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.L63T+p.I93L	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, L63T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.M36I+p.N37D+p.R57G+p.L63T+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, M36I, N37D, R57G, L63T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.N37D+p.G48GV+p.I54IT+p.R57G+p.L63LP+p.H69HR+p.A71X+p.V77VI+p.V82VA+p.I93IL	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, N37D, G48GV, I54IT, R57G, L63LP, H69HR, A71X, V77VI, V82VA, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I54IM+p.L63P+p.V77VI+p.V82A+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I54IM, L63P, V77VI, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I54M+p.L63P+p.H69HY+p.A71V+p.G73GS+p.V82A+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I54M, L63P, H69HY, A71V, G73GS, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K14E+p.L63P+p.I64V+p.E65K+p.H69Q+p.I72L+p.V77I+p.I93IL	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K14E, L63P, I64V, E65K, H69Q, I72L, V77I, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K14KR+p.E35D+p.R57K+p.L63P+p.I64V+p.I93L	Missense mutation	0.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K14KR, E35D, R57K, L63P, I64V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K14R+p.I15V+p.L63Q+p.I72V+p.V77I+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K14R, I15V, L63Q, I72V, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K14R+p.I15V+p.R57G+p.L63Q+p.I72V+p.V77I+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K14R, I15V, R57G, L63Q, I72V, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K14R+p.L63X+p.I72V+p.V77I+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K14R, L63X, I72V, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.E35D+p.M36I+p.N37D+p.I54V+p.R57K+p.D60E+p.L63P+p.A71V+p.I72T+p.V77VI+p.V82A+p.I93L	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, E35D, M36I, N37D, I54V, R57K, D60E, L63P, A71V, I72T, V77VI, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.E35D+p.M36I+p.N37S+p.R41K+p.M46V+p.G48V+p.I54T+p.D60E+p.I62IV+p.L63P+p.A71IV+p.I72IT+p.V82A+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, E35D, M36I, N37S, R41K, M46V, G48V, I54T, D60E, I62IV, L63P, A71IV, I72IT, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.M36I+p.I54V+p.I62IMV+p.L63P+p.A71V+p.V82T+p.L90M	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, M36I, I54V, I62IMV, L63P, A71V, V82T, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K43T+p.M46L+p.K55R+p.R57G+p.A71V+p.V82A+p.N83P+p.L90M	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K43T, M46L, K55R, R57G, A71V, V82A, N83P, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L19I+p.K20R+p.E35D+p.M36I+p.R41K+p.M46I+p.I54V+p.L63P+p.I64V+p.G73T+p.V82A+p.L90M+p.I93L+p.C95F	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L19I, K20R, E35D, M36I, R41K, M46I, I54V, L63P, I64V, G73T, V82A, L90M, I93L, C95F.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L19IT+p.G48V+p.I54V+p.I64V+p.V82A+p.L90M	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L19IT, G48V, I54V, I64V, V82A, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L19Q+p.R41K+p.I54V+p.D60E+p.I62V+p.L63PT+p.A71V+p.V82A+p.I93M	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L19Q, R41K, I54V, D60E, I62V, L63PT, A71V, V82A, I93M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L23I+p.L33LI+p.M36I+p.M46I+p.I54V+p.R57G+p.I62IV+p.L63A+p.A71V+p.T74S+p.V77VI+p.I84IV+p.I85IV+p.L90M+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L23I, L33LI, M36I, M46I, I54V, R57G, I62IV, L63A, A71V, T74S, V77VI, I84IV, I85IV, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L24F+p.M46I+p.D60E+p.I62V+p.L63P+p.A71V+p.I72V+p.G73S+p.V77I+p.L90M+p.I93L	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L24F, M46I, D60E, I62V, L63P, A71V, I72V, G73S, V77I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L24I+p.M46L+p.G48V+p.I54V+p.L63P+p.A71V+p.V77I+p.V82A	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L24I, M46L, G48V, I54V, L63P, A71V, V77I, V82A.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L24I+p.R41K+p.M46I+p.I54V+p.I64V+p.V77I+p.V82A	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L24I, R41K, M46I, I54V, I64V, V77I, V82A.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L24I+p.R41K+p.M46I+p.L63P+p.I64V+p.V82A+p.I84V	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L24I, R41K, M46I, L63P, I64V, V82A, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L24LI+p.L33LF+p.M36MIL+p.R41K+p.M46ML+p.G48GV+p.I54IV+p.K55KR+p.R57G+p.Q58E+p.I62IV+p.L63P+p.I64IV+p.G73AT+p.V82VA+p.I84IV+p.L90LM+p.T91TA	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L24LI, L33LF, M36MIL, R41K, M46ML, G48GV, I54IV, K55KR, R57G, Q58E, I62IV, L63P, I64IV, G73AT, V82VA, I84IV, L90LM, T91TA.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L63P+p.I93L	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.M36I+p.G48V+p.I54V+p.I62V+p.L63P+p.T74S+p.V82A+p.C95G	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, M36I, G48V, I54V, I62V, L63P, T74S, V82A, C95G.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.M36I+p.N37D+p.R57K+p.I62IV+p.L63P+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, M36I, N37D, R57K, I62IV, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.M36MI+p.L63A+p.I93L	Missense mutation	0.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, M36MI, L63A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.M46L+p.I62V+p.L63P+p.I85V+p.L90M+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, M46L, I62V, L63P, I85V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.N37D+p.G48V+p.F53L+p.I54V+p.L63P+p.A71T+p.V77I+p.V82A+p.L90M+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, N37D, G48V, F53L, I54V, L63P, A71T, V77I, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.N37D+p.R57K+p.I62V+p.L63P+p.A71T+p.I72IMT+p.G73S+p.V77I+p.I85V+p.I93L	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, N37D, R57K, I62V, L63P, A71T, I72IMT, G73S, V77I, I85V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.R41RK+p.M46I+p.D60E+p.I62V+p.L63P+p.I64V+p.K70R+p.I72E+p.G73C+p.V77I+p.L90M+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, R41RK, M46I, D60E, I62V, L63P, I64V, K70R, I72E, G73C, V77I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.R41RK+p.M46I+p.R57G+p.D60E+p.I62V+p.L63P+p.I64V+p.K70R+p.I72E+p.G73C+p.V77I+p.L90M+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, R41RK, M46I, R57G, D60E, I62V, L63P, I64V, K70R, I72E, G73C, V77I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12E+p.K20I+p.E35D+p.I62V+p.L63P+p.A71V+p.G73S+p.V77I+p.L90M	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12E, K20I, E35D, I62V, L63P, A71V, G73S, V77I, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12P+p.K14R+p.I15IV+p.N37D+p.I62V+p.L63PT+p.C67H+p.A71T+p.V77I+p.Q92QH+p.I93L	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12P, K14R, I15IV, N37D, I62V, L63PT, C67H, A71T, V77I, Q92QH, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12TK+p.K14R+p.L23I+p.L33LV+p.E35D+p.M36V+p.N37H+p.R41K+p.M46I+p.I50L+p.R57K+p.L63P+p.I64V+p.K70R+p.V82A+p.I85V	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12TK, K14R, L23I, L33LV, E35D, M36V, N37H, R41K, M46I, I50L, R57K, L63P, I64V, K70R, V82A, I85V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12V+p.G17D+p.K20M+p.E35G+p.M36I+p.G48V+p.F53L+p.I54V+p.R57K+p.I62V+p.L63P+p.A71V+p.V82A+p.L90M+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12V, G17D, K20M, E35G, M36I, G48V, F53L, I54V, R57K, I62V, L63P, A71V, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IM+p.I13V+p.Q18H+p.N37D+p.M46I+p.K55KR+p.Q61QH+p.L63P+p.A71T+p.I72T+p.V77I+p.N88S+p.L90M+p.I93L	Missense mutation	1	Susceptible	[8]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IM, I13V, Q18H, N37D, M46I, K55KR, Q61QH, L63P, A71T, I72T, V77I, N88S, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.I13IV+p.K20R+p.M36I+p.I54V+p.I62V+p.L63P+p.I64IL+p.A71V+p.I72IV+p.V82AT+p.L90LM	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, I13IV, K20R, M36I, I54V, I62V, L63P, I64IL, A71V, I72IV, V82AT, L90LM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.I13V+p.K14KR+p.I15V+p.L24I+p.M36MI+p.N37E+p.M46I+p.I54V+p.L63P+p.A71VI+p.V82AT+p.I93IL	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, I13V, K14KR, I15V, L24I, M36MI, N37E, M46I, I54V, L63P, A71VI, V82AT, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.L19I+p.M36I+p.N37T+p.R41K+p.K45KR+p.R57G+p.I62M+p.L63P+p.I64M+p.A71V+p.I93L	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, L19I, M36I, N37T, R41K, K45KR, R57G, I62M, L63P, I64M, A71V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LF+p.I13V+p.I15V+p.G16GA+p.K20X+p.M36MI+p.N37T+p.I54IV+p.R57K+p.L63LP+p.I64V+p.K70R+p.V82VA+p.L90LM	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LF, I13V, I15V, G16GA, K20X, M36MI, N37T, I54IV, R57K, L63LP, I64V, K70R, V82VA, L90LM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LF+p.K14KE+p.E35D+p.N37ND+p.R57RK+p.L63P	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LF, K14KE, E35D, N37ND, R57RK, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LF+p.T12E+p.I13V+p.K20KT+p.E35EK+p.M36I+p.N37DE+p.M46MI+p.I62IV+p.I64V+p.L90LM+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LF, T12E, I13V, K20KT, E35EK, M36I, N37DE, M46MI, I62IV, I64V, L90LM, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LFI+p.L19Q+p.K20KR+p.L23LI+p.E35ED+p.M36MI+p.I54IM+p.R57G+p.I62IV+p.L63LP+p.I64V+p.G73GC+p.T74TS+p.V77VI+p.I84IV+p.L90LM+p.I93IL	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LFI, L19Q, K20KR, L23LI, E35ED, M36MI, I54IM, R57G, I62IV, L63LP, I64V, G73GC, T74TS, V77VI, I84IV, L90LM, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.E35D+p.M36I+p.I62V+p.L63A+p.V77I	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, E35D, M36I, I62V, L63A, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.I13IV+p.I15V+p.E35ED+p.N37C+p.R41RK+p.I62IL+p.L63LP+p.A71AV+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, I13IV, I15V, E35ED, N37C, R41RK, I62IL, L63LP, A71AV, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.I13IV+p.K14KR+p.R57G+p.L63P+p.H69Q+p.I72IM+p.V77VI+p.I93IM	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, I13IV, K14KR, R57G, L63P, H69Q, I72IM, V77VI, I93IM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.I13V+p.D30N+p.L63P+p.K70R+p.A71T+p.V77I+p.N88D	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, I13V, D30N, L63P, K70R, A71T, V77I, N88D.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.I13V+p.K14KR+p.K20KT+p.E35D+p.M36I+p.R41KN+p.H69K+p.V77VI+p.L89M	Missense mutation	0.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, I13V, K14KR, K20KT, E35D, M36I, R41KN, H69K, V77VI, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.I13V+p.L33LF+p.M36MI+p.N37NS+p.R41K+p.Q58QE+p.Q61N+p.L63LP+p.V82VA+p.N88NS	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, I13V, L33LF, M36MI, N37NS, R41K, Q58QE, Q61N, L63LP, V82VA, N88NS.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.I15V+p.M36I+p.N37D+p.K43R+p.R57RK+p.L63P+p.I72V+p.P79PS	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, I15V, M36I, N37D, K43R, R57RK, L63P, I72V, P79PS.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.L33LF+p.M36I+p.R41K+p.M46MIL+p.L63T+p.V82VI+p.I84V	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, L33LF, M36I, R41K, M46MIL, L63T, V82VI, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.T12PS+p.I15IV+p.K20KM+p.M36MI+p.N37ND+p.K45KR+p.I54IV+p.R57G+p.I62IV+p.L63P+p.A71AT+p.G73X+p.V82X+p.I85IV+p.L90LM	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, T12PS, I15IV, K20KM, M36MI, N37ND, K45KR, I54IV, R57G, I62IV, L63P, A71AT, G73X, V82X, I85IV, L90LM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.T12TA+p.R57G+p.I62IV+p.L63AST+p.V77VI+p.I93L	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, T12TA, R57G, I62IV, L63AST, V77VI, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.T12TP+p.K14KR+p.N37X+p.R41RK+p.R57G+p.I62V+p.L63T+p.I64L+p.C67S+p.V77I+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, T12TP, K14KR, N37X, R41RK, R57G, I62V, L63T, I64L, C67S, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LR+p.Q18H+p.L19LI+p.L33LF+p.M46ML+p.I54IL+p.I62V+p.L63P+p.A71AV+p.I72ILM+p.G73X+p.V77I+p.I84IV+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LR, Q18H, L19LI, L33LF, M46ML, I54IL, I62V, L63P, A71AV, I72ILM, G73X, V77I, I84IV, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LV+p.I13V+p.K20R+p.M36I+p.N37K+p.R41A+p.H69K+p.L89M	Missense mutation	0.6	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LV, I13V, K20R, M36I, N37K, R41A, H69K, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LV+p.I15V+p.M36MI+p.N37X+p.R41K+p.L63S+p.I72T	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LV, I15V, M36MI, N37X, R41K, L63S, I72T.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LV+p.K14KR+p.I64IV	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LV, K14KR, I64IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LV+p.T12P+p.I13V+p.K14KR+p.N37D+p.R57G+p.L63Q+p.I64V+p.E65D	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LV, T12P, I13V, K14KR, N37D, R57G, L63Q, I64V, E65D.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LV+p.T12TKN+p.K14KR+p.I15V+p.L19I+p.R41K+p.R57G+p.L63CS+p.E65ED+p.V77I+p.I93IL	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LV, T12TKN, K14KR, I15V, L19I, R41K, R57G, L63CS, E65ED, V77I, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LV+p.T12TS+p.K14NS+p.I15IV+p.L19LI+p.N37ND+p.R41RK+p.K45KR+p.D60DE+p.L63LP+p.I64V	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LV, T12TS, K14NS, I15IV, L19LI, N37ND, R41RK, K45KR, D60DE, L63LP, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10R+p.V32I+p.N37H+p.R41K+p.M46I+p.I47V+p.I50L+p.L63P+p.A71I+p.I72V+p.V77I+p.V82T+p.L90M+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10R, V32I, N37H, R41K, M46I, I47V, I50L, L63P, A71I, I72V, V77I, V82T, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.E35D+p.N37D+p.M46I+p.K55KR+p.L63P+p.A71V+p.G73S+p.V77I+p.L90M+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, E35D, N37D, M46I, K55KR, L63P, A71V, G73S, V77I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13IV+p.L33V+p.E35D+p.L63P+p.R87RG	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13IV, L33V, E35D, L63P, R87RG.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.K14R+p.G16E+p.L19I+p.K20IT+p.E35D+p.M36I+p.P39T+p.F53FL+p.I54V+p.I62V+p.I66F+p.H69K+p.A71AV+p.T74S+p.L89M	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, K14R, G16E, L19I, K20IT, E35D, M36I, P39T, F53FL, I54V, I62V, I66F, H69K, A71AV, T74S, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.K14R+p.L19P+p.K20I+p.M36I+p.R41K+p.L63P+p.H69K+p.K70R+p.I72M+p.L89M	Missense mutation	1.1	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, K14R, L19P, K20I, M36I, R41K, L63P, H69K, K70R, I72M, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.K20R+p.D30N+p.L33F+p.E35D+p.M36I+p.N37D+p.R41K+p.I54V+p.R57G+p.L63P+p.I64V+p.N88D+p.L90M+p.I93L	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, K20R, D30N, L33F, E35D, M36I, N37D, R41K, I54V, R57G, L63P, I64V, N88D, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.K20T+p.L33F+p.M46I+p.Q58E+p.I62V+p.L63P+p.V77I+p.N88G	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, K20T, L33F, M46I, Q58E, I62V, L63P, V77I, N88G.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.L33V+p.M36MI+p.R41K+p.I64V+p.R87RK	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, L33V, M36MI, R41K, I64V, R87RK.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.N37S+p.M46I+p.L63P+p.A71T+p.V77I+p.L90M	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, N37S, M46I, L63P, A71T, V77I, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I15V+p.L19I+p.E35D+p.N37D+p.R57G+p.L63P+p.I64L+p.H69Y	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I15V, L19I, E35D, N37D, R57G, L63P, I64L, H69Y.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I15V+p.M36I+p.N37D+p.R41K+p.L63S+p.I72T	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I15V, M36I, N37D, R41K, L63S, I72T.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.L33IV+p.M46L+p.I54V+p.D60E+p.L63P+p.A71V+p.V82T+p.I84V+p.L90M+p.I93L	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, L33IV, M46L, I54V, D60E, L63P, A71V, V82T, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.M36I+p.K43T+p.G48V+p.I54V+p.I62V+p.I64V+p.T74S+p.V82A	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, M36I, K43T, G48V, I54V, I62V, I64V, T74S, V82A.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.M46I+p.I62V+p.I64V+p.K70R+p.T74A+p.V77I+p.L90M+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, M46I, I62V, I64V, K70R, T74A, V77I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.T12R+p.I15V+p.K20T+p.N37D+p.M46I+p.K55KR+p.I62V+p.L63P+p.A71T+p.G73S+p.T74S+p.V77I+p.T80TA+p.V82T+p.I85V+p.L90M+p.I93L+p.C95F	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, T12R, I15V, K20T, N37D, M46I, K55KR, I62V, L63P, A71T, G73S, T74S, V77I, T80TA, V82T, I85V, L90M, I93L, C95F.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.T12S+p.L19Q+p.L23I+p.M46I+p.I54L+p.K55R+p.L63P+p.A71IV+p.G73S+p.V77I+p.V82T+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, T12S, L19Q, L23I, M46I, I54L, K55R, L63P, A71IV, G73S, V77I, V82T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10X+p.E35D+p.M36I+p.N37E+p.R41RK+p.R57RK+p.I62IV+p.I64IV+p.H69HY+p.I84IV	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10X, E35D, M36I, N37E, R41RK, R57RK, I62IV, I64IV, H69HY, I84IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10Y+p.G16E+p.V32I+p.L33LI+p.E35D+p.M36I+p.N37V+p.M46V+p.I47V+p.F53L+p.K55KR+p.R57G+p.L63A+p.I93L	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10Y, G16E, V32I, L33LI, E35D, M36I, N37V, M46V, I47V, F53L, K55KR, R57G, L63A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19I+p.E35D+p.R57G+p.L63A	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19I, E35D, R57G, L63A.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19I+p.L63P+p.A71AT+p.I72E	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19I, L63P, A71AT, I72E.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19I+p.L63S+p.E65D+p.I72V+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19I, L63S, E65D, I72V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19I+p.M36I+p.N37D+p.R41K+p.R57G+p.Q61E+p.I64V+p.E65ED+p.C67CW	Missense mutation	0.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19I, M36I, N37D, R41K, R57G, Q61E, I64V, E65ED, C67CW.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19I+p.M36I+p.N37D+p.R41K+p.R57G+p.Y59H+p.Q61E+p.I62V+p.L63T+p.I64V+p.A71V+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19I, M36I, N37D, R41K, R57G, Y59H, Q61E, I62V, L63T, I64V, A71V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19I+p.M36MT+p.I62IV+p.L63LP+p.I72M+p.V77VI	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19I, M36MT, I62IV, L63LP, I72M, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19I+p.R57K+p.L63S+p.I64V+p.E65D	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19I, R57K, L63S, I64V, E65D.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19IV+p.M36MI+p.R57G+p.I64V	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19IV, M36MI, R57G, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19LI+p.N37S	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19LI, N37S.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19LP+p.R41K+p.L63P+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19LP, R41K, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19LV+p.Q61E+p.L63LP+p.I64IV	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19LV, Q61E, L63LP, I64IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19Q+p.E35D+p.M36MI+p.N37NS+p.R41K+p.K55R+p.R57RK+p.L63P+p.V75VI	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19Q, E35D, M36MI, N37NS, R41K, K55R, R57RK, L63P, V75VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19Q+p.K20T+p.D30N+p.L33V+p.R57G+p.A71V+p.N88D	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19Q, K20T, D30N, L33V, R57G, A71V, N88D.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19Q+p.M36I+p.N37C+p.I62V+p.L63P+p.I64V	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19Q, M36I, N37C, I62V, L63P, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19Q+p.R57G+p.D60DE+p.L63LP+p.I64V+p.K70KR	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19Q, R57G, D60DE, L63LP, I64V, K70KR.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L19T+p.N37D+p.K43R+p.R57G+p.L63X	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L19T, N37D, K43R, R57G, L63X.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L23I+p.R57K+p.L63P+p.H69Y+p.A71V+p.N88S+p.Q92R+p.I93L	Missense mutation	0.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L23I, R57K, L63P, H69Y, A71V, N88S, Q92R, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L23LI+p.V32VI+p.I50IL+p.I62V+p.L63P+p.A71AV+p.G73CS+p.V77I+p.L90M+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L23LI, V32VI, I50IL, I62V, L63P, A71AV, G73CS, V77I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L33F+p.M36L+p.P39Q+p.R41K+p.K43R+p.I54V+p.I62V+p.L63P+p.A71V+p.V82L+p.I93L	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L33F, M36L, P39Q, R41K, K43R, I54V, I62V, L63P, A71V, V82L, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L33I+p.I62V+p.L63T+p.I64L+p.A71V+p.I72T+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L33I, I62V, L63T, I64L, A71V, I72T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L33LV+p.N37C+p.P39PS+p.L63P+p.H69HY	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L33LV, N37C, P39PS, L63P, H69HY.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L33V+p.N37C+p.R41K+p.R57G+p.L63P+p.I64V+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L33V, N37C, R41K, R57G, L63P, I64V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L38I+p.K43R+p.I62V+p.L63P+p.V77I+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L38I, K43R, I62V, L63P, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L63P+p.H69Q+p.K70R+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L63P, H69Q, K70R, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L63P+p.I64L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L63P, I64L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L63P+p.I72T+p.V77I+p.I93L	Missense mutation	0.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L63P, I72T, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L63P+p.I72T	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L63P, I72T.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L63P+p.K70R	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L63P, K70R.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L63P+p.K70T+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L63P, K70T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L63P+p.V77I+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L63P, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L63P	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L63PS+p.V77VI	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L63PS, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L63T+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L63T, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36I+p.I62V+p.L63A+p.I72IM	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36I, I62V, L63A, I72IM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36I+p.I62V+p.L63P+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36I, I62V, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36I+p.N37D+p.K43KR+p.R57K+p.D60E+p.Q61QE+p.I62IV+p.L63P+p.A71T+p.I72IV+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36I, N37D, K43KR, R57K, D60E, Q61QE, I62IV, L63P, A71T, I72IV, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36I+p.R41K+p.I93L	Missense mutation	0.4	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36I, R41K, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36I+p.R41K+p.R57G+p.I64V	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36I, R41K, R57G, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36I+p.R57G+p.D60DE+p.Q61E+p.I62V+p.L63P+p.A71AT+p.V77VI+p.I93L	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36I, R57G, D60DE, Q61E, I62V, L63P, A71AT, V77VI, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36I+p.R57K+p.L63P+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36I, R57K, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36MI+p.I62V+p.L63P+p.I64L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36MI, I62V, L63P, I64L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36MI+p.K45R+p.R57G+p.I62M+p.L63P+p.A71T+p.I72E+p.I93IM	Missense mutation	0.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36MI, K45R, R57G, I62M, L63P, A71T, I72E, I93IM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36MI+p.N37S+p.R41K+p.L63LP+p.I64IV+p.I72IV+p.V77VI	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36MI, N37S, R41K, L63LP, I64IV, I72IV, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36MI+p.Q58E+p.I62IV+p.L63X+p.I64IV+p.K70R+p.V77VI+p.L90LM	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36MI, Q58E, I62IV, L63X, I64IV, K70R, V77VI, L90LM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M36MI+p.R41RK+p.I62IV+p.V77VI	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M36MI, R41RK, I62IV, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37D+p.D60E+p.I62V+p.L63P+p.G68E+p.V77I+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37D, D60E, I62V, L63P, G68E, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37D+p.R57G+p.L63P+p.V77I+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37D, R57G, L63P, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37E+p.L63P+p.K70T+p.I72R	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37E, L63P, K70T, I72R.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37NS+p.G48GE+p.Q61E+p.L63S+p.E65D+p.I72V+p.V77I	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37NS, G48GE, Q61E, L63S, E65D, I72V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37NS+p.R41RK	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37NS, R41RK.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37S+p.L63T+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37S, L63T, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37S+p.R41K+p.R57G+p.D60DE+p.L63P	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37S, R41K, R57G, D60DE, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37S+p.R41K+p.R57G+p.L63A+p.Q92K	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37S, R41K, R57G, L63A, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37S+p.R41RK+p.R57G+p.L63A+p.Q92QK	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37S, R41RK, R57G, L63A, Q92QK.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37S+p.R57G+p.Q61E+p.L63P+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37S, R57G, Q61E, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37T+p.R57G+p.L63P	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37T, R57G, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.N37Y+p.R41K+p.R57K+p.V77I+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included N37Y, R41K, R57K, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.NULL	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included NULL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q18E+p.R57G+p.I62IV+p.L63S+p.I72V+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q18E, R57G, I62IV, L63S, I72V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q18H+p.R41K+p.L63PS+p.A71V+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q18H, R41K, L63PS, A71V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q18L+p.E35D+p.M36I+p.I62IV+p.L63P+p.K70KR	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q18L, E35D, M36I, I62IV, L63P, K70KR.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q61E+p.I62V+p.L63T+p.E65D+p.V77I	Missense mutation	0.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q61E, I62V, L63T, E65D, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q7QR+p.L10LV+p.I13IV+p.K14R+p.E35D+p.N37DE+p.P39PS+p.R57K+p.I62IV+p.L63P+p.I64V	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q7QR, L10LV, I13IV, K14R, E35D, N37DE, P39PS, R57K, I62IV, L63P, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.Q7R+p.T12S+p.K14R+p.R41K+p.I62V+p.L63P+p.I64M	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included Q7R, T12S, K14R, R41K, I62V, L63P, I64M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R41K+p.D60E+p.I64V+p.V77I	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R41K, D60E, I64V, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R41K+p.L63P+p.V77I	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R41K, L63P, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R41K+p.R57G+p.I62V+p.L63P+p.V77I+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R41K, R57G, I62V, L63P, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R41K+p.R57G+p.L63P+p.T74TA+p.V77I+p.L90LM+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R41K, R57G, L63P, T74TA, V77I, L90LM, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R41RK+p.H69HY	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R41RK, H69HY.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R41RK+p.I62IV+p.L63A+p.V77I	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R41RK, I62IV, L63A, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G+p.I62V+p.L63P+p.V77I+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G, I62V, L63P, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G+p.K70R	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G, K70R.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G+p.L63H+p.V77I+p.L89I+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G, L63H, V77I, L89I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G+p.L63LP+p.I64IV+p.K70KR+p.V77VI	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G, L63LP, I64IV, K70KR, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G+p.L63P+p.I64IL	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G, L63P, I64IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G+p.L63P+p.I64L+p.A71T+p.I72T+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G, L63P, I64L, A71T, I72T, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G+p.L63P	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G+p.L63S+p.V77VI+p.L89M	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G, L63S, V77VI, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57K+p.D60E+p.L63P+p.I64V+p.G73C+p.V77I+p.L90M	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57K, D60E, L63P, I64V, G73C, V77I, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57K+p.I62IV+p.L63AP+p.I64IM+p.H69HY+p.K70KR+p.A71AT	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57K, I62IV, L63AP, I64IM, H69HY, K70KR, A71AT.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57K+p.L63A+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57K, L63A, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57K+p.L63P+p.E65D	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57K, L63P, E65D.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57K+p.L63P+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57K, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57K+p.L63Q	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57K, L63Q.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57K+p.V77I	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57K, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57K+p.Y59YH+p.L63P+p.I72IL	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57K, Y59YH, L63P, I72IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57RK+p.L63P+p.E65ED+p.A71T+p.V77I+p.I93L	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57RK, L63P, E65ED, A71T, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R8RQ+p.T12S+p.I15IV+p.L19I+p.N37S+p.I62IV+p.L63X+p.V77VI	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R8RQ, T12S, I15IV, L19I, N37S, I62IV, L63X, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12A+p.I13V+p.L63P+p.I64V+p.V77VI	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12A, I13V, L63P, I64V, V77VI.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12A+p.I13V+p.L63P	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12A, I13V, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12A+p.I15V+p.G16E+p.P39S+p.R57G+p.L63P+p.A71T+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12A, I15V, G16E, P39S, R57G, L63P, A71T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12A+p.I15V+p.L19I+p.R41K+p.L63P+p.V77I+p.V82L+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12A, I15V, L19I, R41K, L63P, V77I, V82L, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12A+p.I15V+p.R41K+p.I62V+p.L63P+p.V77I+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12A, I15V, R41K, I62V, L63P, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12A+p.K14KR+p.M36I+p.R57K+p.I62V+p.L63P+p.I93L	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12A, K14KR, M36I, R57K, I62V, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12A+p.K14R+p.R57K+p.L63P+p.I72V+p.V77I+p.L89I+p.I93L	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12A, K14R, R57K, L63P, I72V, V77I, L89I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12A+p.L19I+p.L63P+p.I64IV	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12A, L19I, L63P, I64IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12A+p.N37E+p.R57G+p.I62V+p.L63P+p.A71T+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12A, N37E, R57G, I62V, L63P, A71T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12AS+p.I15IV+p.L19LI+p.M36I+p.N37D+p.I62V+p.L63P+p.I72IM	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12AS, I15IV, L19LI, M36I, N37D, I62V, L63P, I72IM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12E+p.I15V+p.L19I+p.E35D+p.M36I+p.R41K+p.I62V+p.L63PS+p.A71AT+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12E, I15V, L19I, E35D, M36I, R41K, I62V, L63PS, A71AT, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12E+p.K14KR+p.K43KR+p.R57K+p.L63P+p.A71T	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12E, K14KR, K43KR, R57K, L63P, A71T.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12I+p.G16E+p.P39S+p.L63T+p.V77I	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12I, G16E, P39S, L63T, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12I+p.I15V+p.E35D+p.N37D+p.R57RK+p.L63P	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12I, I15V, E35D, N37D, R57RK, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12I+p.N37D+p.K45KR+p.R57G+p.L63Q+p.V77I+p.I93L	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12I, N37D, K45KR, R57G, L63Q, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12K+p.I13V+p.L19I+p.M36IV+p.R41K+p.R57G+p.I62V+p.L63P+p.I72IM	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12K, I13V, L19I, M36IV, R41K, R57G, I62V, L63P, I72IM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12LS+p.I15V+p.L19I+p.M36I+p.R41K+p.R57G+p.I62V+p.H69K+p.A71T+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12LS, I15V, L19I, M36I, R41K, R57G, I62V, H69K, A71T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12N+p.K14R+p.I15V+p.G16E+p.K20M+p.M36I+p.P39Q+p.L63S+p.I64V+p.K70R	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12N, K14R, I15V, G16E, K20M, M36I, P39Q, L63S, I64V, K70R.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12N+p.L19I+p.L63P+p.C67D+p.I72MT+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12N, L19I, L63P, C67D, I72MT, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12N+p.L63P+p.H69Q+p.V77I+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12N, L63P, H69Q, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12P+p.I13V+p.K14R+p.E35D+p.N37S+p.Q61E+p.L63P+p.I64IV+p.A71AT+p.I72IV+p.I93IL	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12P, I13V, K14R, E35D, N37S, Q61E, L63P, I64IV, A71AT, I72IV, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12P+p.I13V+p.K14R+p.L19V+p.K20R+p.M36I+p.R41K+p.R57G+p.L63Q+p.I64V+p.A71V+p.V82M+p.I84V+p.I93L	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12P, I13V, K14R, L19V, K20R, M36I, R41K, R57G, L63Q, I64V, A71V, V82M, I84V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12P+p.K14KR+p.N37S+p.R41K+p.R57G+p.L63P+p.I64IV+p.H69HQ+p.A71AT+p.I72L+p.V77I+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12P, K14KR, N37S, R41K, R57G, L63P, I64IV, H69HQ, A71AT, I72L, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12P+p.K14R+p.E35D+p.N37DE+p.R57G+p.L63P+p.A71T+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12P, K14R, E35D, N37DE, R57G, L63P, A71T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12P+p.K14R+p.L63P+p.G68E+p.A71T	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12P, K14R, L63P, G68E, A71T.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12P+p.K14R+p.N37S+p.R41K+p.R57G+p.L63P+p.I64IV+p.H69HQ+p.A71AT+p.I72L+p.V77I+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12P, K14R, N37S, R41K, R57G, L63P, I64IV, H69HQ, A71AT, I72L, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12P+p.K20R+p.V32I+p.E35D+p.M36I+p.N37S+p.R41K+p.L63P+p.I64V+p.C67CW+p.A71V+p.I85IV+p.I93L	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12P, K20R, V32I, E35D, M36I, N37S, R41K, L63P, I64V, C67CW, A71V, I85IV, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12P+p.L19I+p.R57G+p.L63P+p.C67D+p.V77I	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12P, L19I, R57G, L63P, C67D, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12P+p.Q61E+p.I62V+p.L63P+p.C67Y+p.V77I+p.I93L	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12P, Q61E, I62V, L63P, C67Y, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12Q+p.I13V+p.K20KR+p.L63Q+p.I64V+p.I72IMV+p.V77I	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12Q, I13V, K20KR, L63Q, I64V, I72IMV, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12R+p.I15V+p.L19I+p.R41K+p.H69Y	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12R, I15V, L19I, R41K, H69Y.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12S+p.I13V+p.K14KR+p.L63P	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12S, I13V, K14KR, L63P.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12S+p.I15V+p.L19I+p.M36I+p.N37D+p.L63V+p.H69K+p.T74A+p.L89M+p.I93L	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12S, I15V, L19I, M36I, N37D, L63V, H69K, T74A, L89M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12S+p.I15V+p.L19I+p.M36I+p.R41K+p.L63V+p.H69K+p.K70R+p.P79PT+p.L89M	Missense mutation	0.9	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12S, I15V, L19I, M36I, R41K, L63V, H69K, K70R, P79PT, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12S+p.I15V+p.L19IV+p.M36I+p.R41K+p.R57G+p.L63P+p.H69K+p.L89M+p.I93L	Missense mutation	1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12S, I15V, L19IV, M36I, R41K, R57G, L63P, H69K, L89M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12S+p.I62V+p.L63P+p.V77I+p.L90M+p.I93L	Missense mutation	1.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12S, I62V, L63P, V77I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12S+p.K14R+p.R41K+p.I62V+p.L63P+p.I64M	Missense mutation	0.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12S, K14R, R41K, I62V, L63P, I64M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12S+p.L19I+p.M36I+p.R41K+p.L63V+p.H69K+p.T74S+p.V77I+p.L89M+p.I93L	Missense mutation	0.9	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12S, L19I, M36I, R41K, L63V, H69K, T74S, V77I, L89M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12S+p.L19I+p.N37S+p.R57G+p.L63A+p.V77I	Missense mutation	0.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12S, L19I, N37S, R57G, L63A, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12S+p.L19LI+p.L63P+p.I72V+p.V77I+p.I93L	Missense mutation	1.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12S, L19LI, L63P, I72V, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12TA+p.K14KR+p.K20KE+p.E35D+p.R41RK+p.R57G+p.I62V+p.L63P+p.V77VI+p.I93IL	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12TA, K14KR, K20KE, E35D, R41RK, R57G, I62V, L63P, V77VI, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12TK+p.N37X+p.R41RK+p.L63Q	Missense mutation	1.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12TK, N37X, R41RK, L63Q.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12TP+p.I13V+p.M36I+p.R57G+p.I64V	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12TP, I13V, M36I, R57G, I64V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12TS+p.I13V+p.I15IV+p.K20KLM+p.E35D+p.M36I+p.N37D+p.K45R+p.R57G+p.I64V+p.I72IT+p.L90LM	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12TS, I13V, I15IV, K20KLM, E35D, M36I, N37D, K45R, R57G, I64V, I72IT, L90LM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12TS+p.I13V+p.K14KR+p.L19X+p.N37ND+p.L63P+p.V77I	Missense mutation	0.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12TS, I13V, K14KR, L19X, N37ND, L63P, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12V+p.E35D+p.M36I+p.R41K+p.R57K+p.Q61N	Missense mutation	1	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12V, E35D, M36I, R41K, R57K, Q61N.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T4P+p.L10V+p.I13AV+p.L19P+p.K20I+p.E35G+p.M36I+p.N37S+p.R41K+p.M46I+p.I54IV+p.L63LP+p.H69K+p.K70R+p.A71T+p.G73GS+p.L89V+p.L90M	Missense mutation	1.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T4P, L10V, I13AV, L19P, K20I, E35G, M36I, N37S, R41K, M46I, I54IV, L63LP, H69K, K70R, A71T, G73GS, L89V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T4S+p.L10I+p.K20R+p.E35D+p.M36I+p.L63P+p.H69Q+p.L90M	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T4S, L10I, K20R, E35D, M36I, L63P, H69Q, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V11VI+p.N37S+p.R41K+p.L63PS+p.V77I	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V11VI, N37S, R41K, L63PS, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V11VI+p.T12TA+p.I13IV+p.L19I+p.K20I+p.D30N+p.E35N+p.M36I+p.N37DE+p.F53FL+p.I54IV+p.Q58QE+p.D60E+p.Q61E+p.L63P+p.A71V+p.I72T+p.N83ND+p.N88D+p.L90M	Missense mutation	0.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V11VI, T12TA, I13IV, L19I, K20I, D30N, E35N, M36I, N37DE, F53FL, I54IV, Q58QE, D60E, Q61E, L63P, A71V, I72T, N83ND, N88D, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V77I+p.I93L	Missense mutation	0.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.W6P+p.R41K+p.R57G+p.I62V+p.C67S+p.H69Y+p.V77I	Missense mutation	0.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included W6P, R41K, R57G, I62V, C67S, H69Y, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.I15V+p.R41K+p.F53FL+p.I64V+p.H69Y	Missense mutation	1.7	Susceptible	[7]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, I15V, R41K, F53FL, I64V, H69Y.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13IV+p.V32VI+p.L33F+p.E35D+p.M36MI+p.R41K+p.I47IV+p.K55N+p.R57K+p.L63P+p.I64M+p.A71AV+p.L90M+p.I93IL	Missense mutation	5.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13IV, V32VI, L33F, E35D, M36MI, R41K, I47IV, K55N, R57K, L63P, I64M, A71AV, L90M, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.I15V+p.K20I+p.E35Q+p.M36I+p.N37D+p.R41K+p.R57K+p.C67E+p.H69K+p.V82I+p.L89M	Missense mutation	1.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, I15V, K20I, E35Q, M36I, N37D, R41K, R57K, C67E, H69K, V82I, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K20M+p.L33I+p.E35D+p.N37A+p.K43R+p.R57K+p.L63H+p.L89I	Missense mutation	1.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K20M, L33I, E35D, N37A, K43R, R57K, L63H, L89I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.K20T+p.E35N+p.M36I+p.N37E+p.I62V+p.L63P+p.K70R+p.A71I+p.T74S+p.L90M+p.I93L	Missense mutation	1.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, K20T, E35N, M36I, N37E, I62V, L63P, K70R, A71I, T74S, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.V32I+p.L33F+p.E34Q+p.M46I+p.I47V+p.F53L+p.I54V+p.K55KR+p.L63P+p.A71V+p.V82A+p.L90M+p.I93L	Missense mutation	5.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, V32I, L33F, E34Q, M46I, I47V, F53L, I54V, K55KR, L63P, A71V, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I13V+p.V32I+p.L33F+p.M36L+p.K43T+p.M46V+p.I54V+p.K55R+p.R57RK+p.L63P+p.I64V+p.A71V+p.V82A+p.N83D+p.L90M	Missense mutation	3.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I13V, V32I, L33F, M36L, K43T, M46V, I54V, K55R, R57RK, L63P, I64V, A71V, V82A, N83D, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.G16GE+p.L19V+p.M36L+p.N37ND+p.R57K+p.L63LP	Missense mutation	1.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, G16GE, L19V, M36L, N37ND, R57K, L63LP.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.G17D+p.K20R+p.E35D+p.M36I+p.R41K+p.R57K+p.Q61N+p.E65D+p.I72T	Missense mutation	1.6	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, G17D, K20R, E35D, M36I, R41K, R57K, Q61N, E65D, I72T.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.K20R+p.V32I+p.M36I+p.M46I+p.I47IV+p.F53L+p.I54V+p.L63P+p.A71I+p.V82A+p.L90M+p.I93L	Missense mutation	5.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, K20R, V32I, M36I, M46I, I47IV, F53L, I54V, L63P, A71I, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.K20T+p.D30N+p.E35D+p.M36I+p.K43KT+p.R57G+p.Q58E+p.I62V+p.L63P+p.V77I+p.N88D	Missense mutation	1.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, K20T, D30N, E35D, M36I, K43KT, R57G, Q58E, I62V, L63P, V77I, N88D.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I15V+p.K20T+p.L33F+p.E35N+p.M36IL+p.N37ND+p.R41KN+p.M46L+p.I50L+p.R57K+p.Q58E+p.I62V+p.L63P+p.I64V+p.A71V+p.T74S+p.V82M+p.L90M+p.I93L	Missense mutation	3.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I15V, K20T, L33F, E35N, M36IL, N37ND, R41KN, M46L, I50L, R57K, Q58E, I62V, L63P, I64V, A71V, T74S, V82M, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.I3IV+p.L10I+p.V11VI+p.I13V+p.I15V+p.K20M+p.E35G+p.M36I+p.I54V+p.R57G+p.D60E+p.I62V+p.L63P+p.A71V+p.N88D+p.L90M	Missense mutation	2.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included I3IV, L10I, V11VI, I13V, I15V, K20M, E35G, M36I, I54V, R57G, D60E, I62V, L63P, A71V, N88D, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.K20KR+p.V32I+p.E35D+p.M36I+p.K43T+p.M46I+p.R57G+p.L63P+p.A71V+p.V82A+p.I84IV+p.L90LM	Missense mutation	2.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included K20KR, V32I, E35D, M36I, K43T, M46I, R57G, L63P, A71V, V82A, I84IV, L90LM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10C+p.T12K+p.I13V+p.K20T+p.V32I+p.L33V+p.M36I+p.K43I+p.M46I+p.I47V+p.I62IV+p.L63P+p.A71V+p.V82T+p.I84V+p.L89V+p.L90LM+p.I93L	Missense mutation	5.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10C, T12K, I13V, K20T, V32I, L33V, M36I, K43I, M46I, I47V, I62IV, L63P, A71V, V82T, I84V, L89V, L90LM, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.E35D+p.L63P+p.A71V+p.T74P+p.I84V+p.L90M+p.I93L	Missense mutation	1.6	Susceptible	[8]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, E35D, L63P, A71V, T74P, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13IM+p.K14KR+p.K20I+p.L24I+p.E35D+p.M36I+p.R41K+p.M46L+p.G48GA+p.I54V+p.K55KR+p.D60E+p.I62V+p.L63P+p.I64IV+p.A71V+p.V82A+p.I84V+p.T91TS	Missense mutation	4.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13IM, K14KR, K20I, L24I, E35D, M36I, R41K, M46L, G48GA, I54V, K55KR, D60E, I62V, L63P, I64IV, A71V, V82A, I84V, T91TS.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.G16A+p.L19IT+p.K20T+p.M46I+p.I54V+p.R57G+p.L63P+p.A71V+p.I72L+p.G73S+p.I84V+p.L90M+p.I93L	Missense mutation	7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, G16A, L19IT, K20T, M46I, I54V, R57G, L63P, A71V, I72L, G73S, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.I15V+p.D30N+p.L33F+p.E35D+p.M36L+p.N37D+p.K45I+p.F53FL+p.I54V+p.I62V+p.L63P+p.V82A+p.N88D+p.I93L	Missense mutation	4.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, I15V, D30N, L33F, E35D, M36L, N37D, K45I, F53FL, I54V, I62V, L63P, V82A, N88D, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.I15V+p.K20V+p.L33F+p.E35D+p.M36I+p.N37E+p.M46I+p.I54V+p.R57G+p.Q61QE+p.I62V+p.L63P+p.A71AT+p.I72IL+p.G73CS+p.V75VI+p.V82A+p.L90M	Missense mutation	5.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, I15V, K20V, L33F, E35D, M36I, N37E, M46I, I54V, R57G, Q61QE, I62V, L63P, A71AT, I72IL, G73CS, V75VI, V82A, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.L19P+p.D30N+p.L33F+p.E35D+p.M36I+p.K43T+p.M46I+p.I54V+p.K55R+p.R57K+p.I62V+p.L63P+p.A71T+p.I72IM+p.V75I+p.V82A+p.N88D+p.L89V+p.Q92K	Missense mutation	7.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, L19P, D30N, L33F, E35D, M36I, K43T, M46I, I54V, K55R, R57K, I62V, L63P, A71T, I72IM, V75I, V82A, N88D, L89V, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.L24I+p.L33F+p.M36MI+p.M46I+p.L63P+p.A71V+p.P79D+p.V82T+p.I93L	Missense mutation	1.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, L24I, L33F, M36MI, M46I, L63P, A71V, P79D, V82T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.L33F+p.E34Q+p.K43T+p.M46L+p.I54V+p.L63P+p.K70KR+p.A71AV+p.T74S+p.V77I+p.P79S+p.I84V+p.N88D+p.L89M+p.L90M+p.T91S+p.I93L	Missense mutation	8.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, L33F, E34Q, K43T, M46L, I54V, L63P, K70KR, A71AV, T74S, V77I, P79S, I84V, N88D, L89M, L90M, T91S, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.L33I+p.M36I+p.R41K+p.M46L+p.I54L+p.L63P+p.I64V+p.N88D	Missense mutation	2.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, L33I, M36I, R41K, M46L, I54L, L63P, I64V, N88D.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.N37ND+p.K45KR+p.M46I+p.R57G+p.I62IV+p.L63P+p.K70R+p.A71IT+p.I72T+p.G73S+p.V82T+p.I84V+p.I93L	Missense mutation	1.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, N37ND, K45KR, M46I, R57G, I62IV, L63P, K70R, A71IT, I72T, G73S, V82T, I84V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.I13V+p.V32I+p.L33I+p.M36I+p.I54V+p.L63P+p.A71I+p.I85V+p.L90M	Missense mutation	8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, I13V, V32I, L33I, M36I, I54V, L63P, A71I, I85V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.M46I+p.R57K+p.I62V+p.L63P+p.A71T+p.I84V+p.L90M+p.I93L	Missense mutation	1.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, M46I, R57K, I62V, L63P, A71T, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.T12Q+p.I13M+p.K20I+p.M46I+p.I54V+p.Q58E+p.L63P+p.I64V+p.C67FL+p.H69R+p.G73T+p.V77I+p.V82A+p.L90M	Missense mutation	1.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, T12Q, I13M, K20I, M46I, I54V, Q58E, L63P, I64V, C67FL, H69R, G73T, V77I, V82A, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10F+p.T12TK+p.K20I+p.M36I+p.N37D+p.R57K+p.I62V+p.L63P+p.G73C+p.I84V+p.I85V+p.L90M	Missense mutation	2.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10F, T12TK, K20I, M36I, N37D, R57K, I62V, L63P, G73C, I84V, I85V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10FI+p.I13IV+p.K14KR+p.G16GA+p.D30DN+p.L33LF+p.N37NH+p.M46MI+p.I54V+p.R57K+p.L63P+p.G68GE+p.K70KE+p.A71AV+p.V82A+p.N88ND+p.Q92QK+p.I93IL+p.G94X	Missense mutation	1.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10FI, I13IV, K14KR, G16GA, D30DN, L33LF, N37NH, M46MI, I54V, R57K, L63P, G68GE, K70KE, A71AV, V82A, N88ND, Q92QK, I93IL, G94X.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10FI+p.V11VI+p.I13V+p.I15V+p.Q18QH+p.K20I+p.M36I+p.N37D+p.M46L+p.I54M+p.L63P+p.A71IV+p.V82A+p.L90M+p.I93L	Missense mutation	3.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10FI, V11VI, I13V, I15V, Q18QH, K20I, M36I, N37D, M46L, I54M, L63P, A71IV, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.A22V+p.R57G+p.L63P+p.A71V+p.I72L+p.G73S+p.V77I+p.I84V+p.I85V+p.L90M	Missense mutation	1.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, A22V, R57G, L63P, A71V, I72L, G73S, V77I, I84V, I85V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.D30N+p.L33F+p.M46L+p.F53L+p.I54L+p.Q58E+p.L63P+p.A71T+p.G73S+p.V77I+p.N88D	Missense mutation	1.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, D30N, L33F, M46L, F53L, I54L, Q58E, L63P, A71T, G73S, V77I, N88D.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.E34Q+p.G48V+p.I54S+p.I62V+p.L63P+p.A71V+p.T74S+p.V77I+p.V82A+p.I93L	Missense mutation	1.4	Susceptible	[3]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, E34Q, G48V, I54S, I62V, L63P, A71V, T74S, V77I, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.E35D+p.M36I+p.G48V+p.I50V+p.I54V+p.I62V+p.L63A+p.A71V+p.V82T	Missense mutation	2.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, E35D, M36I, G48V, I50V, I54V, I62V, L63A, A71V, V82T.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.E35D+p.M36I+p.N37S+p.R41K+p.R57G+p.I62V+p.L63P+p.A71V+p.G73S+p.L90M+p.I93L	Missense mutation	1.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, E35D, M36I, N37S, R41K, R57G, I62V, L63P, A71V, G73S, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.E35D+p.M36I+p.R41K+p.I62V+p.L63P+p.A71V+p.T74P+p.L90M+p.I93L	Missense mutation	1.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, E35D, M36I, R41K, I62V, L63P, A71V, T74P, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.G16A+p.L33F+p.K45R+p.I54L+p.K55R+p.I62V+p.L63P+p.C67CY+p.I72L+p.G73C+p.V77I+p.I84V+p.L90M+p.I93L	Missense mutation	9.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, G16A, L33F, K45R, I54L, K55R, I62V, L63P, C67CY, I72L, G73C, V77I, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13IV+p.L24F+p.L33F+p.E35D+p.M36I+p.N37ND+p.R41RK+p.K43KT+p.M46MI+p.I54V+p.K55KR+p.I62V+p.L63P+p.A71V+p.I72IV+p.G73S+p.V82A+p.L90M+p.I93L	Missense mutation	3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13IV, L24F, L33F, E35D, M36I, N37ND, R41RK, K43KT, M46MI, I54V, K55KR, I62V, L63P, A71V, I72IV, G73S, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13IV+p.V32I+p.E35D+p.M36MI+p.N37S+p.K43T+p.M46I+p.I47V+p.L63P+p.V77VI+p.V82T+p.I85V	Missense mutation	2.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13IV, V32I, E35D, M36MI, N37S, K43T, M46I, I47V, L63P, V77VI, V82T, I85V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.G16A+p.Q18H+p.L33F+p.N37D+p.M46I+p.I54V+p.Q61H+p.I62IV+p.L63P+p.A71L+p.I72T+p.L76V+p.V77I+p.V82VA+p.N88G+p.L90M+p.I93L	Missense mutation	3	Susceptible	[9]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, G16A, Q18H, L33F, N37D, M46I, I54V, Q61H, I62IV, L63P, A71L, I72T, L76V, V77I, V82VA, N88G, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.I15IV+p.Q18H+p.K20V+p.A22AV+p.L33LIV+p.M36I+p.M46MI+p.I54V+p.K55KR+p.R57K+p.I62V+p.L63P+p.C67W+p.A71V+p.I72L+p.G73S+p.I84V+p.I85IV+p.L90M	Missense mutation	5.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, I15IV, Q18H, K20V, A22AV, L33LIV, M36I, M46MI, I54V, K55KR, R57K, I62V, L63P, C67W, A71V, I72L, G73S, I84V, I85IV, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K14R+p.G16E+p.K20I+p.E35D+p.M36I+p.N37D+p.R41N+p.I54V+p.L63H+p.H69K+p.K70R+p.T74S+p.L76V+p.V82A+p.L89I+p.T91S	Missense mutation	1.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K14R, G16E, K20I, E35D, M36I, N37D, R41N, I54V, L63H, H69K, K70R, T74S, L76V, V82A, L89I, T91S.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20I+p.E35Q+p.M36I+p.R41K+p.R57K+p.L63S+p.C67E+p.H69K+p.V82I+p.L89M	Missense mutation	3.3	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20I, E35Q, M36I, R41K, R57K, L63S, C67E, H69K, V82I, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20I+p.L33V+p.E35D+p.M36I+p.N37T+p.M46I+p.I54V+p.Q58QE+p.I62V+p.L63P+p.I64IV+p.A71V+p.T74P+p.V82L+p.L90M+p.I93L	Missense mutation	6.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20I, L33V, E35D, M36I, N37T, M46I, I54V, Q58QE, I62V, L63P, I64IV, A71V, T74P, V82L, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.K20R+p.L33F+p.E34KR+p.E35D+p.M36I+p.G40GE+p.R41K+p.K43T+p.G48M+p.I54S+p.I62V+p.I72IMT+p.V82A	Missense mutation	2.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, K20R, L33F, E34KR, E35D, M36I, G40GE, R41K, K43T, G48M, I54S, I62V, I72IMT, V82A.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L33F+p.E35D+p.K43T+p.I54L+p.L63P+p.I64L+p.V77I+p.L90M	Missense mutation	3.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L33F, E35D, K43T, I54L, L63P, I64L, V77I, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L33F+p.K43T+p.M46I+p.G48V+p.F53Y+p.I54S+p.R57G+p.D60E+p.Q61E+p.I62V+p.L63T+p.A71V+p.I72V+p.T74P+p.V82A+p.L89M+p.I93L	Missense mutation	2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L33F, K43T, M46I, G48V, F53Y, I54S, R57G, D60E, Q61E, I62V, L63T, A71V, I72V, T74P, V82A, L89M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L33F+p.R41K+p.M46L+p.I54V+p.K55R+p.Q58E+p.D60E+p.Q61E+p.L63P+p.A71T+p.I72T+p.G73C+p.V82A+p.I84V+p.L90M	Missense mutation	9.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L33F, R41K, M46L, I54V, K55R, Q58E, D60E, Q61E, L63P, A71T, I72T, G73C, V82A, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.L33I+p.E35G+p.M36I+p.R41K+p.M46MI+p.I54V+p.K55KR+p.R57RK+p.Q58E+p.D60E+p.L63P+p.A71T+p.I72IV+p.G73S+p.I84V+p.L90M+p.I93L	Missense mutation	4.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, L33I, E35G, M36I, R41K, M46MI, I54V, K55KR, R57RK, Q58E, D60E, L63P, A71T, I72IV, G73S, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I13V+p.N37ND+p.R41K+p.M46I+p.I47V+p.L63P+p.A71T+p.I72V+p.V77I+p.N88G+p.L90M	Missense mutation	3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I13V, N37ND, R41K, M46I, I47V, L63P, A71T, I72V, V77I, N88G, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.G16E+p.L19I+p.K20R+p.L24I+p.E35D+p.M36I+p.I54V+p.I62V+p.L63C+p.A71V+p.V82T+p.I84V	Missense mutation	1.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, G16E, L19I, K20R, L24I, E35D, M36I, I54V, I62V, L63C, A71V, V82T, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.I54L+p.I62V+p.L63P+p.I93L	Missense mutation	3.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, I54L, I62V, L63P, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.K20M+p.L63P+p.A71V+p.G73S+p.P79AD+p.I84V+p.R87RK+p.L90M+p.I93L	Missense mutation	2.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, K20M, L63P, A71V, G73S, P79AD, I84V, R87RK, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.K20T+p.E35D+p.M36I+p.M46I+p.I54V+p.K55KR+p.R57RK+p.Q58E+p.D60DE+p.I62V+p.L63P+p.A71T+p.I72IT+p.N83ND+p.I85V+p.N88S+p.L90M	Missense mutation	2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, K20T, E35D, M36I, M46I, I54V, K55KR, R57RK, Q58E, D60DE, I62V, L63P, A71T, I72IT, N83ND, I85V, N88S, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.L19I+p.K20R+p.E35D+p.M36I+p.N37E+p.R41K+p.I54V+p.Q58E+p.D60N+p.I62V+p.L63P+p.K70R+p.A71V+p.G73S+p.V82A+p.L90M+p.I93L	Missense mutation	1.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, L19I, K20R, E35D, M36I, N37E, R41K, I54V, Q58E, D60N, I62V, L63P, K70R, A71V, G73S, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.L19I+p.K20R+p.M36I+p.R41K+p.M46L+p.I54V+p.L63P+p.A71V+p.L76V+p.V82A+p.L90M+p.I93L	Missense mutation	2.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, L19I, K20R, M36I, R41K, M46L, I54V, L63P, A71V, L76V, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.I15V+p.L24I+p.M46L+p.I54V+p.L63P+p.A71V+p.V77I+p.V82A+p.I84V	Missense mutation	2.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, I15V, L24I, M46L, I54V, L63P, A71V, V77I, V82A, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K14KR+p.G16GE+p.G17GE+p.R57K+p.L63T+p.A71T+p.I93L	Missense mutation	1.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K14KR, G16GE, G17GE, R57K, L63T, A71T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K14R+p.I15IV+p.E35D+p.M36I+p.R41K+p.M46L+p.I54V+p.Q58E+p.I62V+p.L63P+p.C67F+p.H69HR+p.A71V+p.I72KR+p.G73S+p.V82A+p.L90M+p.C95L	Missense mutation	2.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K14R, I15IV, E35D, M36I, R41K, M46L, I54V, Q58E, I62V, L63P, C67F, H69HR, A71V, I72KR, G73S, V82A, L90M, C95L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K14R+p.V32I+p.R41K+p.G48M+p.F53L+p.I54M+p.I62V+p.L63A+p.C67D+p.H69R+p.A71L+p.I72V+p.V77I+p.V82A+p.I84V+p.L90M+p.I93L	Missense mutation	9.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K14R, V32I, R41K, G48M, F53L, I54M, I62V, L63A, C67D, H69R, A71L, I72V, V77I, V82A, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20I+p.E35D+p.M36I+p.M46I+p.I62V+p.L63P+p.I64V+p.A71V+p.I72V+p.L76V+p.L90M+p.I93L	Missense mutation	1.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20I, E35D, M36I, M46I, I62V, L63P, I64V, A71V, I72V, L76V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.E35D+p.M36I+p.K43T+p.I47V+p.G48M+p.F53L+p.I54V+p.I62V+p.L63P+p.I66F+p.A71V+p.V82T+p.I84V+p.Q92R	Missense mutation	3.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, E35D, M36I, K43T, I47V, G48M, F53L, I54V, I62V, L63P, I66F, A71V, V82T, I84V, Q92R.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.E35D+p.M36I+p.N37D+p.M46L+p.G48V+p.I54V+p.D60E+p.I62V+p.L63P+p.A71V+p.I72V+p.V77I+p.V82S+p.L90M+p.I93L	Missense mutation	3.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, E35D, M36I, N37D, M46L, G48V, I54V, D60E, I62V, L63P, A71V, I72V, V77I, V82S, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.E35D+p.M36I+p.N37D+p.R41K+p.M46I+p.I54V+p.I62V+p.L63P+p.V82A+p.L90M+p.I93L+p.C95F	Missense mutation	2.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, E35D, M36I, N37D, R41K, M46I, I54V, I62V, L63P, V82A, L90M, I93L, C95F.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.L24I+p.E35D+p.M36I+p.N37E+p.M46I+p.I54V+p.Q61N+p.I62V+p.L63P+p.I64V+p.A71V+p.I72V+p.T74S+p.L76V+p.V82T+p.I93L	Missense mutation	3.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, L24I, E35D, M36I, N37E, M46I, I54V, Q61N, I62V, L63P, I64V, A71V, I72V, T74S, L76V, V82T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.L24I+p.E35D+p.N37DE+p.M46I+p.I54V+p.R57RK+p.Q61H+p.I62V+p.L63P+p.I64V+p.A71V+p.I72V+p.T74S+p.L76V+p.V82T+p.I93L	Missense mutation	3.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, L24I, E35D, N37DE, M46I, I54V, R57RK, Q61H, I62V, L63P, I64V, A71V, I72V, T74S, L76V, V82T, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.L24I+p.L33F+p.E35D+p.M36I+p.N37D+p.M46L+p.I54V+p.L63P+p.A71T+p.V82A+p.T91S	Missense mutation	3.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, L24I, L33F, E35D, M36I, N37D, M46L, I54V, L63P, A71T, V82A, T91S.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.V32I+p.E35D+p.M36I+p.R41RK+p.K43T+p.M46I+p.I47V+p.F53FL+p.I54V+p.K55R+p.L63P+p.A71V+p.V82A+p.L90M	Missense mutation	5.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, V32I, E35D, M36I, R41RK, K43T, M46I, I47V, F53FL, I54V, K55R, L63P, A71V, V82A, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.K20R+p.V32I+p.L33F+p.E34K+p.E35D+p.M36V+p.N37S+p.M46I+p.I47V+p.G48E+p.I54M+p.R57K+p.Q58E+p.I62V+p.L63P+p.A71T+p.V77T+p.I84V+p.L90M+p.I93L	Missense mutation	5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, K20R, V32I, L33F, E34K, E35D, M36V, N37S, M46I, I47V, G48E, I54M, R57K, Q58E, I62V, L63P, A71T, V77T, I84V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L19I+p.K20M+p.V32I+p.L33LF+p.M36I+p.M46I+p.I47V+p.F53L+p.I54V+p.I62V+p.L63A+p.I64IM+p.A71V+p.V82A+p.L90M	Missense mutation	4.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L19I, K20M, V32I, L33LF, M36I, M46I, I47V, F53L, I54V, I62V, L63A, I64IM, A71V, V82A, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L24I+p.L33F+p.N37S+p.M46L+p.I54V+p.K55KR+p.I62IV+p.L63P+p.A71V+p.I72IM+p.G73GCS+p.V82A	Missense mutation	2.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L24I, L33F, N37S, M46L, I54V, K55KR, I62IV, L63P, A71V, I72IM, G73GCS, V82A.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L33F+p.G48V+p.I54S+p.Q58QE+p.D60E+p.L63P+p.A71V+p.V77I+p.V82A+p.T91A	Missense mutation	4.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L33F, G48V, I54S, Q58QE, D60E, L63P, A71V, V77I, V82A, T91A.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.L33LF+p.E34Q+p.M46MIV+p.G48V+p.I50V+p.I54S+p.I62V+p.L63P+p.A71V+p.I72V+p.T74S+p.V77I+p.V82A+p.I93L	Missense mutation	5.8	Susceptible	[3]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, L33LF, E34Q, M46MIV, G48V, I50V, I54S, I62V, L63P, A71V, I72V, T74S, V77I, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.N37S+p.M46I+p.I62V+p.L63P+p.A71V+p.T74P+p.V77I+p.I84V+p.L90M+p.Q92R+p.I93L	Missense mutation	4.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, N37S, M46I, I62V, L63P, A71V, T74P, V77I, I84V, L90M, Q92R, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.Q18QH+p.L24LF+p.L33F+p.N37X+p.I54V+p.Q58E+p.L63P+p.A71I+p.I72L+p.G73AT+p.V77I+p.V82F+p.L90M+p.I93L	Missense mutation	7.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, Q18QH, L24LF, L33F, N37X, I54V, Q58E, L63P, A71I, I72L, G73AT, V77I, V82F, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12I+p.N37D+p.M46L+p.G48V+p.R57K+p.L63Q+p.I64V+p.A71V+p.I72T+p.V82A+p.I84V	Missense mutation	2.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12I, N37D, M46L, G48V, R57K, L63Q, I64V, A71V, I72T, V82A, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12K+p.I15V+p.N37H+p.R41RK+p.K55N+p.L63P+p.A71V+p.V77I+p.I84V+p.I85V+p.N88D+p.L90M	Missense mutation	2.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12K, I15V, N37H, R41RK, K55N, L63P, A71V, V77I, I84V, I85V, N88D, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12TK+p.E35D+p.M36MI+p.M46I+p.I54V+p.I62V+p.L63P+p.I64IV+p.G73GC+p.T74S+p.V82A+p.L90M+p.I93L+p.C95F	Missense mutation	2	Susceptible	[3]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12TK, E35D, M36MI, M46I, I54V, I62V, L63P, I64IV, G73GC, T74S, V82A, L90M, I93L, C95F.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.T12TK+p.L33F+p.K43T+p.M46I+p.I50L+p.I54V+p.K55R+p.I62V+p.L63P+p.A71V+p.I72L+p.G73T+p.V77I+p.V82A+p.L90M+p.I93L+p.C95F	Missense mutation	1.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, T12TK, L33F, K43T, M46I, I50L, I54V, K55R, I62V, L63P, A71V, I72L, G73T, V77I, V82A, L90M, I93L, C95F.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.I13IV+p.E35D+p.M36I+p.R41K+p.M46L+p.L63P+p.A71V+p.I84V+p.L90M+p.Q92K+p.I93L	Missense mutation	2.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, I13IV, E35D, M36I, R41K, M46L, L63P, A71V, I84V, L90M, Q92K, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.I13V+p.G16A+p.L33F+p.N37D+p.M46L+p.I54V+p.R57G+p.I62V+p.L63P+p.I72L+p.G73S+p.V77I+p.V82A+p.L90M+p.I93L	Missense mutation	4.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, I13V, G16A, L33F, N37D, M46L, I54V, R57G, I62V, L63P, I72L, G73S, V77I, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.I13V+p.I15IV+p.Q18H+p.K20I+p.N37NT+p.M46I+p.I54V+p.L63P+p.A71V+p.I72M+p.G73S+p.I84V+p.L90M	Missense mutation	7.4	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, I13V, I15IV, Q18H, K20I, N37NT, M46I, I54V, L63P, A71V, I72M, G73S, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11I+p.T12TP+p.I13V+p.K20KR+p.E35D+p.M36I+p.R41K+p.M46L+p.I62IV+p.L63P+p.A71V+p.I84V+p.L90M+p.Q92K+p.I93L	Missense mutation	4.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11I, T12TP, I13V, K20KR, E35D, M36I, R41K, M46L, I62IV, L63P, A71V, I84V, L90M, Q92K, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V11L+p.I13V+p.I15V+p.Q18H+p.K20I+p.E35G+p.M36I+p.N37D+p.I54V+p.L63P+p.A71V+p.I72L+p.G73GS+p.I84V+p.L90M	Missense mutation	8.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V11L, I13V, I15V, Q18H, K20I, E35G, M36I, N37D, I54V, L63P, A71V, I72L, G73GS, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V32I+p.M46I+p.I47A+p.I62V+p.L63P+p.V77I+p.V82I+p.Q92K+p.I93L+p.C95F	Missense mutation	9.3	Susceptible	[3]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V32I, M46I, I47A, I62V, L63P, V77I, V82I, Q92K, I93L, C95F.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10I+p.V32VI+p.L33LF+p.E34EQ+p.I47IV+p.I54IM+p.D60E+p.L63P+p.K70T+p.A71AV+p.I72IK+p.G73GS+p.V77I+p.L90LM+p.I93L	Missense mutation	4.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10I, V32VI, L33LF, E34EQ, I47IV, I54IM, D60E, L63P, K70T, A71AV, I72IK, G73GS, V77I, L90LM, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.I13V+p.G16A+p.L33F+p.R41RK+p.M46L+p.I54V+p.Q58E+p.I62V+p.L63P+p.I66F+p.A71V+p.I72L+p.G73S+p.V77I+p.P79PA+p.V82T+p.I84IV+p.L90M	Missense mutation	2.2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, I13V, G16A, L33F, R41RK, M46L, I54V, Q58E, I62V, L63P, I66F, A71V, I72L, G73S, V77I, P79PA, V82T, I84IV, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.I13V+p.G16E+p.K20R+p.D30N+p.L33F+p.E35D+p.M36I+p.N37T+p.K45R+p.I54V+p.R57K+p.Q58E+p.I62IV+p.L63T+p.E65D+p.I72V+p.V82A+p.N88D	Missense mutation	2.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, I13V, G16E, K20R, D30N, L33F, E35D, M36I, N37T, K45R, I54V, R57K, Q58E, I62IV, L63T, E65D, I72V, V82A, N88D.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.I13V+p.I15IV+p.K20R+p.L33F+p.E34D+p.E35D+p.M36I+p.K45R+p.I54V+p.R57K+p.I62V+p.L63P+p.I66V+p.A71V+p.G73S+p.V82A+p.I84V+p.I85L+p.L90M+p.I93L	Missense mutation	3.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, I13V, I15IV, K20R, L33F, E34D, E35D, M36I, K45R, I54V, R57K, I62V, L63P, I66V, A71V, G73S, V82A, I84V, I85L, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.I13V+p.L33F+p.E34ED+p.M36MV+p.M46L+p.I54V+p.Q58E+p.D60E+p.I62V+p.L63P+p.I66IV+p.K70KT+p.A71M+p.I72IM+p.V77I+p.V82T+p.I84V+p.L89M+p.I93L	Missense mutation	5.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, I13V, L33F, E34ED, M36MV, M46L, I54V, Q58E, D60E, I62V, L63P, I66IV, K70KT, A71M, I72IM, V77I, V82T, I84V, L89M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.I13V+p.L33I+p.E35D+p.M36I+p.I47IM+p.I54V+p.Q58QE+p.I62V+p.L63P+p.A71V+p.V82F+p.I85IV+p.L89V+p.L90M+p.I93L	Missense mutation	5.1	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, I13V, L33I, E35D, M36I, I47IM, I54V, Q58QE, I62V, L63P, A71V, V82F, I85IV, L89V, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.K14R+p.I15V+p.M36I+p.G48V+p.I54V+p.D60E+p.I62V+p.I64V+p.T74A+p.V82A+p.I84V	Missense mutation	2.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, K14R, I15V, M36I, G48V, I54V, D60E, I62V, I64V, T74A, V82A, I84V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10IV+p.V32VI+p.M46I+p.I47V+p.L63P+p.V77I+p.Q92QKR+p.I93L+p.C95F	Missense mutation	2.7	Susceptible	[8]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10IV, V32VI, M46I, I47V, L63P, V77I, Q92QKR, I93L, C95F.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LF+p.G16A+p.L19I+p.G27GE+p.L33F+p.N37NS+p.M46I+p.G51A+p.K55R+p.Q58E+p.D60E+p.L63P+p.A71V+p.I72IM+p.G73S+p.V77I+p.V82A+p.L90M	Missense mutation	5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LF, G16A, L19I, G27GE, L33F, N37NS, M46I, G51A, K55R, Q58E, D60E, L63P, A71V, I72IM, G73S, V77I, V82A, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LFV+p.L33LI+p.N37NH+p.M46I+p.I54V+p.D60E+p.I62V+p.L63P+p.I64IV+p.I66V+p.I72IT+p.G73T+p.V77I+p.I84V+p.I85V+p.L90M+p.I93L+p.C95V	Missense mutation	4.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LFV, L33LI, N37NH, M46I, I54V, D60E, I62V, L63P, I64IV, I66V, I72IT, G73T, V77I, I84V, I85V, L90M, I93L, C95V.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.I15IV+p.G17DE+p.K20IMR+p.R41RK+p.I62IV+p.L63P+p.A71V+p.G73X+p.V77VI+p.I84IV+p.I85IV+p.L90M+p.I93L	Missense mutation	0.6	Susceptible	[3]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, I15IV, G17DE, K20IMR, R41RK, I62IV, L63P, A71V, G73X, V77VI, I84IV, I85IV, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.I15V+p.G16GE+p.K20R+p.E35D+p.M36I+p.R41K+p.I54IV+p.R57K+p.Q61N+p.L63LH+p.I64IM+p.K70KR+p.L89M+p.L90LM	Missense mutation	2	Susceptible	[3]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, I15V, G16GE, K20R, E35D, M36I, R41K, I54IV, R57K, Q61N, L63LH, I64IM, K70KR, L89M, L90LM.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.L19LI+p.M36MI+p.N37NS+p.R41K+p.I54IV+p.Q58QE+p.I62V+p.L63DN+p.A71AV+p.I72IT+p.V82VA+p.I93IL	Missense mutation	1.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, L19LI, M36MI, N37NS, R41K, I54IV, Q58QE, I62V, L63DN, A71AV, I72IT, V82VA, I93IL.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.L19LI+p.V32VI+p.L33F+p.N37T+p.M46MI+p.I50IL+p.I54IV+p.I62IMV+p.I64IV+p.A71AV+p.V82VA+p.I84IV	Missense mutation	1.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, L19LI, V32VI, L33F, N37T, M46MI, I50IL, I54IV, I62IMV, I64IV, A71AV, V82VA, I84IV.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.L24I+p.R41K+p.M46L+p.I54V+p.I62V+p.L63P+p.A71V+p.T74A+p.V77I+p.V82A+p.I93L	Missense mutation	0.4	Susceptible	[3]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, L24I, R41K, M46L, I54V, I62V, L63P, A71V, T74A, V77I, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.T12PS+p.L19I+p.V32I+p.E35D+p.M36I+p.R41RK+p.M46ML+p.I47A+p.Q61QH+p.L63P+p.I64V+p.A71AV+p.I93L	Missense mutation	1.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, T12PS, L19I, V32I, E35D, M36I, R41RK, M46ML, I47A, Q61QH, L63P, I64V, A71AV, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10LI+p.V32VI+p.L33M+p.R41K+p.K43KR+p.M46I+p.I62V+p.L63P+p.C67CF+p.A71IT+p.I72V+p.G73GC+p.T74P+p.V77I+p.L90M+p.I93L	Missense mutation	2.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10LI, V32VI, L33M, R41K, K43KR, M46I, I62V, L63P, C67CF, A71IT, I72V, G73GC, T74P, V77I, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10M+p.I13V+p.K14R+p.K20I+p.E35Q+p.M36I+p.R41K+p.R57K+p.C67E+p.H69K+p.V82VI+p.L89M	Missense mutation	2	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10M, I13V, K14R, K20I, E35Q, M36I, R41K, R57K, C67E, H69K, V82VI, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13IV+p.I15V+p.L19I+p.V32I+p.L33LF+p.M46I+p.I47A+p.K55KN+p.Q58QE+p.L63P+p.K70KE+p.A71V+p.V77I+p.V82A+p.I93L	Missense mutation	7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13IV, I15V, L19I, V32I, L33LF, M46I, I47A, K55KN, Q58QE, L63P, K70KE, A71V, V77I, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.Q18H+p.L19V+p.K20R+p.L33I+p.M36I+p.N37S+p.K45KR+p.I62IV+p.L63P+p.I64V+p.A71AV+p.N83ND+p.I84V+p.T91TS+p.Q92K	Missense mutation	3.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, Q18H, L19V, K20R, L33I, M36I, N37S, K45KR, I62IV, L63P, I64V, A71AV, N83ND, I84V, T91TS, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I13V+p.Q18H+p.L19V+p.K20R+p.L33I+p.M36I+p.N37S+p.R57G+p.L63P+p.I64V+p.I84V+p.Q92K	Missense mutation	1.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I13V, Q18H, L19V, K20R, L33I, M36I, N37S, R57G, L63P, I64V, I84V, Q92K.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.I15V+p.E35D+p.M36I+p.R41K+p.R57K+p.Q61DN+p.L63CS+p.I93L	Missense mutation	1.5	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, I15V, E35D, M36I, R41K, R57K, Q61DN, L63CS, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.K20R+p.L33F+p.M36I+p.M46L+p.I54LV+p.L63P+p.A71V+p.V82A+p.L90M+p.I93L	Missense mutation	2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, K20R, L33F, M36I, M46L, I54LV, L63P, A71V, V82A, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.L23I+p.L24M+p.N37NH+p.K43KT+p.M46I+p.F53L+p.I54V+p.K55R+p.D60E+p.I62V+p.L63P+p.A71V+p.I72V+p.G73ST+p.V77I+p.L90M	Missense mutation	5.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, L23I, L24M, N37NH, K43KT, M46I, F53L, I54V, K55R, D60E, I62V, L63P, A71V, I72V, G73ST, V77I, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.L24I+p.M46I+p.I54V+p.K55KR+p.R57G+p.Q58E+p.D60E+p.L63P+p.I64V+p.I72V+p.V77I+p.V82A	Missense mutation	1.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, L24I, M46I, I54V, K55KR, R57G, Q58E, D60E, L63P, I64V, I72V, V77I, V82A.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L10V+p.N37D+p.K43KT+p.M46MI+p.G48LV+p.F53FY+p.I54S+p.I62V+p.L63P+p.H69Y+p.A71V+p.T74S+p.V77I+p.V82A+p.I93L	Missense mutation	2.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L10V, N37D, K43KT, M46MI, G48LV, F53FY, I54S, I62V, L63P, H69Y, A71V, T74S, V77I, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L33F+p.K43T+p.L63C+p.H69Q+p.V77I	Missense mutation	2.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L33F, K43T, L63C, H69Q, V77I.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.L33F+p.M36L+p.P39Q+p.R41K+p.K43R+p.M46I+p.I47V+p.F53L+p.I54V+p.I62V+p.L63P+p.A71V+p.V82L+p.I93L	Missense mutation	2.8	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included L33F, M36L, P39Q, R41K, K43R, M46I, I47V, F53L, I54V, I62V, L63P, A71V, V82L, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.M46L+p.K55R+p.L63P+p.H69Y+p.A71T+p.I84V+p.L90M	Missense mutation	3.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included M46L, K55R, L63P, H69Y, A71T, I84V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R41K+p.Q58QH+p.L63P+p.I72IM+p.V77I+p.I93L	Missense mutation	1.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R41K, Q58QH, L63P, I72IM, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.R57G+p.L63Q	Missense mutation	1.6	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included R57G, L63Q.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12N+p.K14R+p.I15V+p.L19IM+p.K20R+p.E35D+p.M36I+p.R41K+p.R57K+p.L63T+p.E65D+p.I72T+p.G73GD+p.T74S+p.L89M	Missense mutation	1.5	Susceptible	[6]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12N, K14R, I15V, L19IM, K20R, E35D, M36I, R41K, R57K, L63T, E65D, I72T, G73GD, T74S, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T12P+p.I13IV+p.K14R+p.L19V+p.K20R+p.M36MI+p.R41K+p.M46I+p.R57G+p.L63HQ+p.I64V+p.V82L+p.I84V+p.I93L	Missense mutation	2	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T12P, I13IV, K14R, L19V, K20R, M36MI, R41K, M46I, R57G, L63HQ, I64V, V82L, I84V, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T4P+p.L10F+p.Q18QH+p.L19I+p.L33F+p.E34Q+p.N37D+p.M46I+p.I54L+p.L63P+p.A71I+p.I72L+p.V77I+p.N88G+p.L90M+p.I93L	Missense mutation	4.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T4P, L10F, Q18QH, L19I, L33F, E34Q, N37D, M46I, I54L, L63P, A71I, I72L, V77I, N88G, L90M, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T4S+p.V11I+p.I13V+p.L19V+p.K20IV+p.L33V+p.E35D+p.M36I+p.N37NS+p.M46IL+p.I54V+p.K55R+p.R57K+p.L63P+p.I64V+p.C67F+p.H69R+p.A71I+p.I72F+p.G73T+p.P79A+p.V82VL+p.I84V+p.L90M+p.I93L+p.T96S	Missense mutation	9.3	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T4S, V11I, I13V, L19V, K20IV, L33V, E35D, M36I, N37NS, M46IL, I54V, K55R, R57K, L63P, I64V, C67F, H69R, A71I, I72F, G73T, P79A, V82VL, I84V, L90M, I93L, T96S.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T4TA+p.L10F+p.I15IV+p.K20KT+p.E35D+p.M46I+p.I54V+p.L63P+p.I64V+p.A71V+p.I72L+p.G73T+p.V77VI+p.I84V+p.I85V+p.L90M	Missense mutation	7.9	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T4TA, L10F, I15IV, K20KT, E35D, M46I, I54V, L63P, I64V, A71V, I72L, G73T, V77VI, I84V, I85V, L90M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.T4TN+p.I13V+p.L33F+p.M36I+p.R41K+p.R57K+p.E65D+p.H69K+p.L89M	Missense mutation	1.7	Susceptible	[7]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included T4TN, I13V, L33F, M36I, R41K, R57K, E65D, H69K, L89M.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V32I+p.I47V+p.Q61E+p.L63P+p.V82A+p.I93L	Missense mutation	0.5	Susceptible	[3]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V32I, I47V, Q61E, L63P, V82A, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.V32I+p.M46I+p.I47V+p.I62V+p.L63P+p.I72Y+p.G73S+p.V77I+p.I93L	Missense mutation	2.5	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included V32I, M46I, I47V, I62V, L63P, I72Y, G73S, V77I, I93L.

Key Molecule: HIV1 Protease (HIV1 PR)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
p.W6WR+p.L10FI+p.I13V+p.M46MI+p.I54IV+p.L63AP+p.C67CF+p.A71T+p.I72IV+p.I84IV+p.N88ND+p.L90LM+p.I93L+p.C95CF	Missense mutation	1.7	Susceptible	[1]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Experiment for
Molecule Alteration

Sanger sequencing assay; GeneSeq assay; Population sequencing of the integrase region assay; DNA sequencing assay; Site-directed mutagenesis

Experiment for
Drug Resistance

PhenoSense assay; Viral load assay; A single-cycle assay; TZM-bl cell line-based phenotypic assay; SDMs and phenotyping assay

Mechanism Description

Some mutations identified were assiacted with resistance in the HIV isolates, those mutations included W6WR, L10FI, I13V, M46MI, I54IV, L63AP, C67CF, A71T, I72IV, I84IV, N88ND, L90LM, I93L, C95CF.

Drug Inactivation by Structure Modification (DISM)

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Key Molecule: Cytochrome P450 family 3 subfamily A (CYP3A)

Molecule Info

Sensitive Disease

Human immunodeficiency virus infection [ICD-11: 1C62.0]

Molecule Alteration

Alteration Patterns	Alteration Types	Resistance Mutation Total Point Scores	Levels of Drug resistance base on the total point score	Ref
Down-regulation	Expression	N.A.	Susceptible	[10]

Sensitive Drug

Darunavir

Drug Info

Experimental Note

Identified from the Human Clinical Data

Mechanism Description

Darunavir, a HIV PI that is co-administered with low-dose ritonavir (darunavir/ritonavir), is recommended in combination with other antiretrovirals. The mechanism of ritonavir pharmacokinetic enhancement is through inhibiting cytochrome P450 (CYP) 3A. Ritonavir has intrinsic antiviral activity against HIV-1 when administered at high doses (i.e., 600 mg twice daily) and was the second PI to be licensed. Along with saquinavir and indinavir, it heralded the advent of highly active antiretroviral therapy.

References

Ref 1	HIV-1 protease mutations and protease inhibitor cross-resistanceAntimicrob Agents Chemother. 2010 Oct;54(10):4253-61. doi: 10.1128/AAC.00574-10. Epub 2010 Jul 26.
Ref 2	HIV-1 Protease and reverse-transcriptase mutations: correlations with antiretroviral therapy in subtype B isolates and implications for drug-resistance surveillanceJ Infect Dis. 2005 Aug 1;192(3):456-65. doi: 10.1086/431601. Epub 2005 Jul 5.
Ref 3	Phenotypic susceptibility to TMC-114 and tipranavir before and after lopinavir/ritonavir-based treatment in subjects demonstrating evolution of lopinavir resistance.
Ref 4	Characterization of HIV-1 drug resistance development through week 48 in antiretroviral naive subjects on rilpivirine/emtricitabine/tenofovir DF or efavirenz/emtricitabine/tenofovir DF in the STaR study (GS-US-264-0110). J Acquir Immune Defic Syndr. 2014 Mar 1;65(3):318-26. doi: 10.1097/QAI.0000000000000017.
Ref 5	Four Amino Acid Changes in HIV-2 Protease Confer Class-Wide Sensitivity to Protease Inhibitors. J Virol. 2015 Nov 11;90(2):1062-9. doi: 10.1128/JVI.01772-15. Print 2016 Jan 15.
Ref 6	Evidence for different susceptibility to tipranavir and darunavir in patients infected with distinct HIV-1 subtypesAIDS. 2008 Mar 12;22(5):611-6. doi: 10.1097/QAD.0b013e3282f51eb9.
Ref 7	Antiretroviral drug susceptibility among drug-naive adults with recent HIV infection in Rakai, UgandaAIDS. 2009 Apr 27;23(7):845-52. doi: 10.1097/QAD.0b013e328327957a.
Ref 8	Identification of genotypic changes in human immunodeficiency virus protease that correlate with reduced susceptibility to the protease inhibitor lopinavir among viral isolates from protease inhibitor-experienced patientsJ Virol. 2001 Aug;75(16):7462-9. doi: 10.1128/JVI.75.16.7462-7469.2001.
Ref 9	Selection of resistance in protease inhibitor-experienced, human immunodeficiency virus type 1-infected subjects failing lopinavir- and ritonavir-based therapy: mutation patterns and baseline correlatesJ Virol. 2005 Mar;79(6):3329-38. doi: 10.1128/JVI.79.6.3329-3338.2005.
Ref 10	Darunavir/cobicistat once daily for the treatment of HIV. Expert Rev Anti Infect Ther. 2015 Jun;13(6):691-704. doi: 10.1586/14787210.2015.1033400.

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Correspondence

Prof. Feng ZHU (zhufeng@zju.edu.cn)