Disease Information
General Information of the Disease (ID: DIS00210)
Name |
Gestational trophoblastic neoplasia
|
---|---|
ICD |
ICD-11: 2C75
|
Resistance Map |
Type(s) of Resistant Mechanism of This Disease
IDUE: Irregularity in Drug Uptake and Drug Efflux
Drug Resistance Data Categorized by Drug
Approved Drug(s)
1 drug(s) in total
Methotrexate
Drug Resistance Data Categorized by Their Corresponding Mechanisms | ||||
Irregularity in Drug Uptake and Drug Efflux (IDUE) | ||||
Key Molecule: SRY-box transcription factor 8 (SOX8) | [1] | |||
Resistant Disease | Gestational trophoblastic neoplasia [ICD-11: 2C75.0] | |||
Molecule Alteration | Expression | Up-regulation |
||
Resistant Drug | Methotrexate | |||
Experimental Note | Identified from the Human Clinical Data | |||
Cell Pathway Regulation | Cell viability | Activation | hsa05200 | |
Cell apoptosis | Inhibition | hsa04210 | ||
In Vitro Model | JEG3 cells | Brain | Homo sapiens (Human) | CVCL_0363 |
Experiment for Molecule Alteration |
RT-PCR | |||
Experiment for Drug Resistance |
CCK-8 assay | |||
Mechanism Description | Over-expression of SOX8 promoted cell survival, enhanced soft agar clonogenesis, and attenuated caspase-3 activities after drug treatment in GTN cells. Importantly, SOX8 might be a potential regulator of reactive oxygen species (ROS) homeostasis, as SOX8 regulated the expression of antioxidant enzymes (GPX1, HMOX1) and reduced drug-induced ROS accumulation in GTN cell models. Collectively, SOX8 might promote drug resistance through attenuating the accumulation of ROS induced by chemotherapeutic drugs in GTN cells. | |||
Key Molecule: SRY-box transcription factor 8 (SOX8) | [1] | |||
Resistant Disease | Gestational trophoblastic neoplasia [ICD-11: 2C75.0] | |||
Molecule Alteration | Expression | Up-regulation |
||
Resistant Drug | Methotrexate | |||
Experimental Note | Identified from the Human Clinical Data | |||
Cell Pathway Regulation | Cell viability | Activation | hsa05200 | |
Cell apoptosis | Inhibition | hsa04210 | ||
In Vitro Model | JEG3 cells | Brain | Homo sapiens (Human) | CVCL_0363 |
Experiment for Molecule Alteration |
RT-PCR | |||
Experiment for Drug Resistance |
CCK-8 assay | |||
Mechanism Description | Over-expression of SOX8 promoted cell survival, enhanced soft agar clonogenesis, and attenuated caspase-3 activities after drug treatment in GTN cells. Importantly, SOX8 might be a potential regulator of reactive oxygen species (ROS) homeostasis, as SOX8 regulated the expression of antioxidant enzymes (GPX1, HMOX1) and reduced drug-induced ROS accumulation in GTN cell models. Collectively, SOX8 might promote drug resistance through attenuating the accumulation of ROS induced by chemotherapeutic drugs in GTN cells. |
Investigative Drug(s)
1 drug(s) in total
Actinomycin D
Drug Resistance Data Categorized by Their Corresponding Mechanisms | ||||
Irregularity in Drug Uptake and Drug Efflux (IDUE) | ||||
Key Molecule: SRY-box transcription factor 8 (SOX8) | [1] | |||
Resistant Disease | Gestational trophoblastic neoplasia [ICD-11: 2C75.0] | |||
Molecule Alteration | Expression | Up-regulation |
||
Resistant Drug | Actinomycin D | |||
Experimental Note | Identified from the Human Clinical Data | |||
Cell Pathway Regulation | Cell viability | Activation | hsa05200 | |
Cell apoptosis | Inhibition | hsa04210 | ||
In Vitro Model | JEG3 cells | Brain | Homo sapiens (Human) | CVCL_0363 |
Experiment for Molecule Alteration |
RT-PCR | |||
Experiment for Drug Resistance |
CCK-8 assay | |||
Mechanism Description | Over-expression of SOX8 promoted cell survival, enhanced soft agar clonogenesis, and attenuated caspase-3 activities after drug treatment in GTN cells. Importantly, SOX8 might be a potential regulator of reactive oxygen species (ROS) homeostasis, as SOX8 regulated the expression of antioxidant enzymes (GPX1, HMOX1) and reduced drug-induced ROS accumulation in GTN cell models. Collectively, SOX8 might promote drug resistance through attenuating the accumulation of ROS induced by chemotherapeutic drugs in GTN cells. | |||
Key Molecule: SRY-box transcription factor 8 (SOX8) | [1] | |||
Resistant Disease | Gestational trophoblastic neoplasia [ICD-11: 2C75.0] | |||
Molecule Alteration | Expression | Up-regulation |
||
Resistant Drug | Actinomycin D | |||
Experimental Note | Identified from the Human Clinical Data | |||
Cell Pathway Regulation | Cell viability | Activation | hsa05200 | |
Cell apoptosis | Inhibition | hsa04210 | ||
In Vitro Model | JEG3 cells | Brain | Homo sapiens (Human) | CVCL_0363 |
Experiment for Molecule Alteration |
RT-PCR | |||
Experiment for Drug Resistance |
CCK-8 assay | |||
Mechanism Description | Over-expression of SOX8 promoted cell survival, enhanced soft agar clonogenesis, and attenuated caspase-3 activities after drug treatment in GTN cells. Importantly, SOX8 might be a potential regulator of reactive oxygen species (ROS) homeostasis, as SOX8 regulated the expression of antioxidant enzymes (GPX1, HMOX1) and reduced drug-induced ROS accumulation in GTN cell models. Collectively, SOX8 might promote drug resistance through attenuating the accumulation of ROS induced by chemotherapeutic drugs in GTN cells. | |||
Key Molecule: SRY-box transcription factor 8 (SOX8) | [1] | |||
Resistant Disease | Gestational trophoblastic neoplasia [ICD-11: 2C75.0] | |||
Molecule Alteration | Expression | Up-regulation |
||
Resistant Drug | Actinomycin D | |||
Experimental Note | Identified from the Human Clinical Data | |||
Cell Pathway Regulation | Cell viability | Activation | hsa05200 | |
Cell apoptosis | Inhibition | hsa04210 | ||
In Vitro Model | JEG3 cells | Brain | Homo sapiens (Human) | CVCL_0363 |
Experiment for Molecule Alteration |
RT-PCR | |||
Experiment for Drug Resistance |
CCK-8 assay | |||
Mechanism Description | Over-expression of SOX8 promoted cell survival, enhanced soft agar clonogenesis, and attenuated caspase-3 activities after drug treatment in GTN cells. Importantly, SOX8 might be a potential regulator of reactive oxygen species (ROS) homeostasis, as SOX8 regulated the expression of antioxidant enzymes (GPX1, HMOX1) and reduced drug-induced ROS accumulation in GTN cell models. Collectively, SOX8 might promote drug resistance through attenuating the accumulation of ROS induced by chemotherapeutic drugs in GTN cells. | |||
Key Molecule: SRY-box transcription factor 8 (SOX8) | [1] | |||
Resistant Disease | Gestational trophoblastic neoplasia [ICD-11: 2C75.0] | |||
Molecule Alteration | Expression | Up-regulation |
||
Resistant Drug | Actinomycin D | |||
Experimental Note | Identified from the Human Clinical Data | |||
Cell Pathway Regulation | Cell viability | Activation | hsa05200 | |
Cell apoptosis | Inhibition | hsa04210 | ||
In Vitro Model | JEG3 cells | Brain | Homo sapiens (Human) | CVCL_0363 |
Experiment for Molecule Alteration |
RT-PCR | |||
Experiment for Drug Resistance |
CCK-8 assay | |||
Mechanism Description | Over-expression of SOX8 promoted cell survival, enhanced soft agar clonogenesis, and attenuated caspase-3 activities after drug treatment in GTN cells. Importantly, SOX8 might be a potential regulator of reactive oxygen species (ROS) homeostasis, as SOX8 regulated the expression of antioxidant enzymes (GPX1, HMOX1) and reduced drug-induced ROS accumulation in GTN cell models. Collectively, SOX8 might promote drug resistance through attenuating the accumulation of ROS induced by chemotherapeutic drugs in GTN cells. |
References
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